Determination of vitamin D3 encapsulated in liprotide β-lactoglobulin crosslink oleic acid by HPLC and SEM

Sylvia Rahmi Putri -  Diponegoro University, Indonesia
Gemala Anjani* -  Diponegoro University, Indonesia
Faizah Fulyani -  Diponegoro University, Indonesia
Diana Nur Afifah -  Diponegoro University, Indonesia
Ahmad Syauqy -  Diponegoro University, Indonesia
Fitriyono Ayustaningwarno -  Diponegoro University, Indonesia
DOI : 10.30867/action.v8i4.1290

Supp. File(s): Surat Pernyataan Etika Publikasi Surat Pernyataan Perjanjian Hak Cipta Surat Pernyataan Covering Letter

Vitamin D is an unstable compound to light, heat, and certain chemicals, so it is easily degraded under various conditions. Therefore, instability is achieved through the encapsulation method using complex protein carrier compounds with fatty acids known as liprotide. This study aims to determine vitamin D3. Method, the determination of vitamin D3 was analyzed, including the determination of vitamin D3 standard eluents, calibration curve, and determining the retention time of vitamin D3 encapsulated in liprotide using HPLC (High-Performance Liquid Chromatography). Morphology of vitamin D3 encapsulated in liprotide using SEM (Scanning Electron Microscopy). The results showed that the standard vitamin D3 HPLC analysis using acetonitrile: methanol and acetonitrile: aquabidest obtained two peaks. Butanol: n-hexane eluent obtained one peak with a retention time of 2,170 minutes. The results of the standard calibration curve for vitamin D3 at a linearity value of R2 = 0,9997 and f(x) = 14,928x-117,930. The same retention time was obtained for vitamin D3 encapsulated in liprotide. Conclusion, an enlargement of the cubic structure, which occurred due to the encapsulation of vitamin D3 by the liprotide-forming micelles.

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Keywords : β-lactoglobulin, HPLC, Liprotide, Oleic acid, SEM, Vitamin D3

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Open Access Copyright (c) 2023 Sylvia Rahmi Putri, Gemala Anjani, Faizah Fulyani, Diana Nur Afifah, Ahmad Syauqy, Fitriyono Ayustaningwarno
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AcTion: Aceh Nutrition Journal
Published by: Department of Nutrition at the Health Polytechnic of Aceh, Ministry of Health.
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