The effect of EKORMIN on rats with diabetes mellitus model: effectiveness on fasting blood glucose, MDA, and insulin levels

Siti Nurjana Kurniaty Tanaiyo* -  Master Program of Nutrition Science of Postgraduate, Universitas Sebelas Maret, Indonesia
Budiyanti Wiboworini -  Department of Nutrition, Faculty of Medicine, Universitas Sebelas Maret, Indonesia
Setyo Sri Rahardjo -  Department of Nutrition, Faculty of Medicine, Universitas Sebelas Maret, Indonesia

Supp. File(s): Research Results Research Instrument Research Instrument common.other
Antioxidants use for additional diabetes mellitus therapy is growing, such us  Okra (Abelmoschus esculentus) and Turmeric (Curcuma longa) with rich flavonoids and beneficial effects on diabetes mellitus. This research aimed to determine the Okra-Turmeric Extract (EKORMIN) on Fasting Blood Glucose (FBG), Malondialdehyde (MDA) and Insulin levels in diabetic rats. The research was conducted in December-January 2022 at PSPG UGM using experimental research with pre-posttest-controlled group design. White male Wistar rats (n = 35) randomly grouped into five: negative control (STZ-NA), positive control (STZ-NA+metformin 1,8 mg/200gBW), P1 (STZ-NA+EKORMIN low dose 130,5;110,5 mg/kgBW), P2 (STZ-NA+EKORMIN moderate dose 261;221 mg/kgBW), P3 (STZ-NA+EKORMIN high dose 522;442 mg/kgBW). The intervention was 14 days. Data analysis used one-way Anova statistical test and post hoc follow-up test. EKORMIN in all doses reduced FBG and MDA, also increased insulin (p<0,05). P2 and P3 were indistinctive from the metformin (PG) group (p>0,05). The decrease in FBG (-167,05±-2,8 vs -175,86±-1,4 mg/dL) and MDA levels (-6,32±0,33 vs -7,98±0,07 nmol/ml) P3 was higher than P2. Similarly for increased insulin levels (121,47±3,03 and 164,09±4,48 pg/ml). EKORMIN was effective in reducing FBG and MDA also increasing insulin in diabetic rat. EKORMIN has anti-diabetic effect and potential for type 2 diabetes mellitus treatment.

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Keywords : Abelmoschus esculentus, curcuma longa, diabetes mellitus, MDA, insulin

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