Effect of dangke administration on serum TNF-a and liver SOD levels in rats induced by a high-fat, high-sugar diet

Uzifatun Nur Y.F Lapatta* -  Master program of nutrition science of postgraduate, faculty of medicine, Diponegoro University, Semarang, Central Java, Indonesia
Diana Nur Afifah -  Department of Nutrition Science, Faculty of Medicine, Diponegoro University, Semarang, Central Java, Indonesia
Ardiyan Pramono -  Department of Nutrition Science, Faculty of Medicine, Diponegoro University, Semarang, Central Java, Indonesia
Ninik Rustanti -  Department of Nutrition Science, Faculty of Medicine, Diponegoro University, Semarang, Central Java, Indonesia
DOI : 10.30867/action.v9i4.1940

Supp. File(s): Research Results Research Results Research Results Research Results
Dangkes contain lactic acid bacteria that improve obesity by controlling the intestinal flora, improving the micro-ecological balance in the gut, and boosting the immune system. This study aimed to analyze the differences in serum TNF-α and liver SOD levels in obese rats fed a Dangke diet. The research was conducted from December to January 2024 at the PSPG PAU UGM using a true-experiment design post-test with only the control group. Male Sprague-Dawley rats (n=30) were randomly divided into five groups: healthy rats (KS), obese rats given standard feeding (K-), obese rats received L-Bio (K+), obese rats were given Dangke at a low dose (P1), and obese rats received Dangke at a high dose (P2). Intervention for 28 days. Data analysis was performed using one-way ANOVA tests and further post-hoc Bonferroni tests.  The result obtainded that Dangke with dose dose of 3,6 g/200 gBB was effective in decreasing TNF-α levels and increasing SOD levels (p<0,05). P2 had the lowest mean TNF-α level (7,33 ± 0,22) compared to K+ (8,59 ± 0,22) and P1 (8,88 ± 0,18). P2 had the highest SOD levels compared to K+ (62,56 ± 4,06) and P1 (61,47 ± 4,71).  In conclusion, Dangke effectively overcame inflammation and oxidative stress in obesity.

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Keywords : Dangke, TNF-α, SOD, oxidative stress, obesity

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Open Access Copyright (c) 2024 Uzifatun Nur Y.F.Lapatta, Diana Nur Afifah, Adriyan Pramono, Ninik Rustanti
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AcTion: Aceh Nutrition Journal
Published by: Department of Nutrition at the Health Polytechnic of Aceh, Ministry of Health.
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