The effetiveness of various sequestrants on the reduction of cadmium and lead levels in Remis (Donax sp.)

In-In Hanidah* -  Program Studi Teknologi Pangan, Fakultas Teknologi Industri Pertanian, Universitas Padjadjaran, Indonesia
Jedi Riazi -  Program Studi Teknologi Pangan, Fakultas Teknologi Industri Pertanian, Universitas Padjadjaran, Indonesia
Tita Rialita -  Program Studi Teknologi Pangan, Fakultas Teknologi Industri Pertanian, Universitas Padjadjaran, Indonesia
Sumanti Debby Moody -  Program Studi Teknologi Pangan, Fakultas Teknologi Industri Pertanian, Universitas Padjadjaran, Indonesia
Yuli Andriani -  Program Studi Perikanan, Fakultas Perikanan dan Ilmu Kelautan, Universitas Padjadjaran, Indonesia
Noor Taufiq -  Pertamina Hulu Energi Offshore North West Java, Jakarta Selatan, Indonesia
Annisa Ratri Utami -  Pertamina Hulu Energi Offshore North West Java, Jakarta Selatan, Indonesia
Laras Aprilianti -  Pertamina Hulu Energi Offshore North West Java, Jakarta Selatan

Supp. File(s): Covering Letters Research Ethics Statement Copyright Agreement

Mussels (Donax sp.) are marine shellfish that tend to be contaminated with heavy metals as they are filter feeders. Heavy metal levels can be reduced by immersion in sequestrants. This study aimed to measure the effectiveness of the optimal sequestrant to reduce heavy metal levels in mussels. This experimental study was conducted at the Food Technology Laboratory of Padjadjaran University in January-April 2024 using samples of mussels (Donax sp.) from Cirebon, Indonesia. The data processing method used was a Randomized Group Design with five treatments consisting of soaking mussels in distilled water, 25% citric acid, 25% tamarind, 25% tomato, and 25% Pidada; each repeated 3x. The results showed that the most optimal organic sequestrant in reducing heavy metals was Pidada fruit with the effectiveness of reducing cadmium metal 21,195%, lead metal 6,543%, total citric acid 0.552%, water content 76.953%, and antioxidant activity based on IC50 value of 19,537 ppm with a very strong category. In conclusion, the Pidada fruit is the most optimal organic sequestrant for reducing heavy metal levels in mussels (Donax sp.) and has very strong antioxidant activity, making it effective for reducing heavy metal contamination.

Supplement Files

Keywords : Cadmium, clams, mangrove, lead, sequestrants

  1. AOAC. (2000). AOAC 942.15-1965 Acidity (Titratable) of Fruit Products : AOAC Official Method. In AOAC Official Methode of analysis (p. Chapter 37, 11).
  2. Badan Standarisasi Nasional. (2011a). SNI 2346:2011 Petunjuk Pengujian Organoleptik dan atau Sensori pada Produk Perikanan. 12.
  3. Badan Standarisasi Nasional. (2011b). SNI 2354.5:2011 Penentuan Kadar Logam Berat Timbal (Pb) dan Kadmium (Cd) Pada Produk Perikanan.
  4. Badan Standarisasi Nasional. (2015). SNI 2354.2:2015 Tentang Cara Uji Kimia Bagian 2: Pengujian Kadar Air pada Produk Perikanan.
  5. Basyuni, M., Siagian, Y. S., Wati, R., Putri, L. A. P., Yusraini, E., & Lesmana, I. (2019a). Fruit nutrition content, hedonic test, and processed products of pidada (Sonneratia caseolaris). IOP Conference Series: Earth and Environmental Science, 251(1). https://doi.org/10.1088/1755-1315/251/1/012042
  6. Basyuni, M., Siagian, Y. S., Wati, R., Putri, L. A. P., Yusraini, E., & Lesmana, I. (2019b). Fruit Nutrition Content, Hedonic Test, and Processed Products of Pidada (Sonneratia caseolaris). IOP Conference Series: Earth and Environmental Science, 251(1). https://doi.org/10.1088/1755-1315/251/1/012042
  7. BPOM. (2024). Cek BTP. Pencarian Global. https://standarpangan.pom.go.id/cekbtp/web/relasi-baru/index?page=13&sort=-keterangan
  8. Cahyo, R. D., Efendi, R., & Herawati, N. (2022). Pengupasan Kulit Buah Dengan Perendaman Air Panas Terhadap Rendemen, Sifat Fisiko-Kimia dan Organoleptik Jus Tomat. JOM Faperta, 9, 1–10.
  9. Devi, B., & Boruah, T. (2020). Antioxidants in Fruits: Properties and Health Benefits. In Antioxidants in Fruits: Properties and Health Benefits (Issue December 2020). https://doi.org/10.1007/978-981-15-7285-2
  10. Edina, L. M., Sumarto, & Edison. (2017). The Effect of Varied Concentration Tamarind (Tamarindus indica L) Extract and The Soaking Time On The Reduction Level of Heavy Metals Plumbum (Pb) and Cadmium (Cd) in The Clam (Anadara granosa) Blood. Teknologi Hasil Perikanan, Cd, 1–12.
  11. Elvira, M. V., Faustino-Eslava, D. V., de Chavez, E. R. C., Losloso, J. A. L., & Fukuyama, M. (2021). Human Health Risk Associated with Heavy Metals from Consumption of Asiatic Clam, Corbicula Fluminea, from Laguna de Bay, Philippines. Environmental Science and Pollution Research, 28(27), 36626–36639. https://doi.org/10.1007/s11356-021-13298-7
  12. Ernawati, Dewi, N. N., & Triastuti, J. (2022). Pengaruh Pemberian Asam Sitrat dengan Konsentrasi yang Berbeda Terhadap Kadar Logam Berat Timbal ( Pb ) pada Daging Kerang Hijau ( Perna viridis ) The Effect of Giving Citric Acid with Different Concentration on The Level of Heavy Metal Lead ( Pb ) in Mea. 11(1), 21–28.
  13. Gümüşay, Ö. A., Borazan, A. A., Ercal, N., & Demirkol, O. (2015). Drying effects on the antioxidant properties of tomatoes and ginger. Food Chemistry, 173, 156–162. https://doi.org/10.1016/j.foodchem.2014.09.162
  14. Gupta, M., Torrico, D. D., Hepworth, G., Gras, S. L., Ong, L., Cottrell, J. J., & Dunshea, F. R. (2021). Differences in Hedonic Responses, Facial Expressions and Self-Reported Emotions of Consumers Using Commercial Yogurts: A Cross-Cultural Study. Foods, 10(1237), 1–17. https://doi.org/10.3390/foods10061237
  15. Hakim, M. I. Al, Silviyati, I., & Supraptiah, E. (2023). Pengaruh Penambahan Ekstrak Asam Jawa (Tamarindus Indica L) Terhadap Kualitas & Kuantitas Piezoelectric Crystal Sebagai Transduser Energi Listrik. 8(2011), 13–28.
  16. Halifah, P., Hartati, Rachmawaty, Yusminah, H., & Roshanida, A. R. (2019). Phytochemical screening and antimicrobial activity from sonneratia caseolaris fruit extract. Materials Science Forum, 967 MSF, 28–33. https://doi.org/10.4028/www.scientific.net/MSF.967.28
  17. Hasanah, N. (2022). Literature Review : Heavy Metal Contamination of Lead ( Pb ) In Local Food Bilvaves. Media Gizi Kesehatan Masyarakat, 11, 589–594.
  18. Indasah, Arsiniati Arbai, Sugijanto, & Sugianto Agus. (2011). Citric Acid Reduces The Content of Pb and Cd of Kupang Beras (Corbula Faba). Folia Medica Indonesiana, 47(1), 46–51.
  19. Jayanti, T. D. W. I., Ilza, M., & Karnila, R. (2018). Pengaruh Konsentrasi Lama Perendaman Berbeda Larutan Belimbing Wuluh (Averrhoa bilimbi L) Terhadap Kandungan Logam Berat (Pb dan Cd) pada Kijing (Pilsbryoconcha exilis). 1–14.
  20. Limesri, L., Nursafitri, E., Ginting, S. B. G., Darni, Y., & Azhar. (2023). Ekstraksi Likopen Dari Buah Tomat (Solanum Lycopersicum) Menggunakan Solvent N-Heksan. Jurnal Redoks, 8(2), 9–16. https://doi.org/10.31851/redoks.v8i2.12774
  21. Mahardhika, R., Riyadi, P. H., & Fahmi, A. S. (2016). Pengaruh Lama Waktu Perendaman Kerang Hijau (Perna Viridis) menggunakan Buat Tomat (Lycoperdicum esculentum) terhadap Penurunan Kadar Logam Timbal (Pb). J. Peng. & Biotek, 5(4), 43–50. http://www.ejournal-s1.undip.ac.id/index.php/ipbhp
  22. Manganti, M., Mandey, L., & Oessoe, Y. (2021). Pemanfaatan Tepung Sagu (Metroxylon sp.) dan Kacang Hijau (Glycine max Merr.) dalam Pembuatan Food Bars. Journal of Food Reserch, 1(1), 44–54.
  23. Mopoung, R., & Kengkhetkit, N. (2016). Lead and cadmium removal efficiency from aqueous solution by NaOH treated pineapple waste. International Journal of Applied Chemistry, 12(1), 23–35.
  24. Nurhadi, B., Suriati, Tensiska, Saputra, R. A., & Sukri, N. (2020). The role of encapsulant materials on the stability of bioactive compounds of red ginger (Zingiber officinale Roscoe. var. Rubrum) extract powder during storage. Food Chemistry, 333, 127490. https://doi.org/10.1016/j.foodchem.2020.127490
  25. Ondu, A. F., Jayadripraja, E. A., & Sunarsih. (2019). Efektifitas Air Perasan Buah Jeruk Nipis (Citrus aurantifolia Cristm. & Panzer Swingle) terhadap Penurunan Kadar Logam Timbal (Pb), Cadmium (Cd), dan Tembaga (Cu) pada Daging Kerang Hijau (Perna viridis L). Higiene, 1(5), 1–13. http://103.55.216.56/index.php/higiene/article/view/9760%0Ahttp://journal.uin-alauddin.ac.id/index.php/higiene/article/view/9760/6774
  26. Parwata, I. M. O. A. (2016). Antioksidan. Kimia Terapan Program Pascasarjana Universitas Udayana, April, 1–54.
  27. Patriani, P., & Apsari, N. (2022). Peningkatan Mutu Daging Menggunakan Rempah (Issue July).
  28. Pomanto, R. M., Dali, F. A., & Mile, L. (2016). Pengaruh Larutan Asam Alami terhadap Mutu Kimiawi Tepung Ikan Manggabai. Jurnal Ilmiah Perikanan Dan Kelautan, 4(3), 1–6.
  29. Požgajová, M., Navrátilová, A., & Kovár, M. (2022). Curative Potential of Substances with Bioactive Properties to Alleviate Cd Toxicity: A Review. International Journal of Environmental Research and Public Health, 19(19), 2–42. https://doi.org/10.3390/ijerph191912380
  30. Putri, C. R. H. (2014). The Potency and Use of Tamarindus indica On Various. Ilmiah Kedokteran, 3, 40–54.
  31. Putri, S. A., Masithah, E. D., & Saputra, E. (2023). The Effect of Citric Acid Soaking Time on The Levels of Lead (Pb) in Lorjuk Meat (Solen sp.). Journal of Marine and Coastal Science, 12(1), 1–9. https://doi.org/10.20473/jmcs.v12i1.37150
  32. Sari, S. Y. P., Ambarwati, R., & Rahayu, D. A. (2021). Molecular characteristics of Donax faba (Bivalvia: Donacidae) from Nepa Beach, Madura, based on cytochrome oxidase subunit I gene sequences. AACL Bioflux, 14(4), 2416–2426.
  33. Suarman, Umroh, & Kurniawan. (2019). Abundance and Pattern of Bean Clams Donax sp. Distribution at Batu Bedaun Beach and Beriga Bangka Belitung. Journal of Aquatropica Asia, 4(1), 26–32.
  34. Susanti, M. M., & Priamsari, M. R. (2016). Pengaruh Perendaman Larutan Tomat ( Solanum lycopersicum L .) Terhadap Penurunan Kadar Logam Berat Timbal ( Pb ) dan Kadmium ( Cd ) Pada Kerang Darah ( Anadara granosa ) The Effect of Soaking Solution Tomato ( Solanum lycopersicum L .) With The Decreasing. International Journal On Medicine Science, 3(2), 11–16.
  35. Susanto, A., Rifkowaty, E. E., Rosmalinda, Kurniawan, T., & Assorudin. (2020). Rekayasa Pembuatan Nanoenkapsulan Ekstrak Buah Pedada (Sonneratia caseolaris) Sebagai Antioksidan Alami dan Sifat Fisikokimia Yang Dihasilkan. 2(2), 97–108.
  36. Swastawati, F., Rizkirana, S. R., Romadhon, Muniroh, M., Mulyono, & Nugraheni, A. (2021). Removal of heavy metals from green mussels (Perna viridis) using pineapple (Ananas comosus) solution as a source of citric acid. IOP Conference Series: Earth and Environmental Science, 919(1). https://doi.org/10.1088/1755-1315/919/1/012041
  37. Sweis, I. E., & Cressey, B. C. (2018). Potential Role of The Common Food Additive Manufactured Citric Acid in Eliciting Significant Inflammatory Reactions Contributing to Serious Disease States: A Series of Four Case Reports. Toxicology Reports, 5(August), 808–812. https://doi.org/10.1016/j.toxrep.2018.08.002
  38. Tari, A. A., Duan, F. K., & Amalo, D. (2018). Analisis Kandungan Gizi Jenis-Jenis Kerang Yang Biasa Dikonsumsi Masyarakat Nembe Desa Oeseli Kecamatan Rote Barat Daya Kabupaten Rote Ndao NTT. Jurnal Biotropikal Sains, 15(2), 1–9.
  39. Tukiran, Miranti, M. G., Wati, I. D., Sofyah, T. M., Rahmawati, T., Fatikhurohmah, H. M., & Sabila, F. I. (2021). Uji Aktivitas Antioksidan Hasil Freeze Dry dari Ekstrak Air Daun Kelor (Moringa oleifera), Umbi Bit (Beta Vulgaris L.), dan Brokoli (Brassica oleracea L.) Sebagai Bahan Tambahan Minuman Suplemen. Prosiding SainsTeKes, 118–126.
  40. Wulandari, D., Ananda, M., & Junita. (2020). Karakteristik Kimia Sari Buah Pedada ( Sonneratia caseolaris ) Selama Penyimpanan. Teknologi Pertanian, 24(1410–1920), 189–195.

Open Access Copyright (c) 2024 In-In Hanidah, Jedi Riazi, Tita Rialita, Sumanti Debby Moody, Yuli Andriani, Noor Taufiq, Annisa Ratri Utami, Laras Aprilianti
Creative Commons License
This work is licensed under a Creative Commons Attribution-ShareAlike 4.0 International License.

AcTion: Aceh Nutrition Journal
Published by: Department of Nutrition at the Health Polytechnic of Aceh, Ministry of Health.
Soekarno-Hatta Street, No. 168. Health Polytechnic of Aceh, Aceh Besar, 23352. Telp/Fax: 0651 46126 / 0651 46121.
Website: https://gizipoltekkesaceh.ac.id/
E-mail: jurnal6121@gmail.com

e-issn: 2548-5741, p-issn: 2527-3310

All content is licensed under a: Creative Commons Attribution ShareAlike 4.0 International License

View My Stats

Get a feed by atom here, RRS2 here and OAI Links here