Comparative of storage time and prebiotic sources (Inulin and Unripe Banana Flour) on the nutritional content of synbiotic yogurt
The consumption of synbiotic yogurt continues to rise; however, Indonesia's reliance on imported inulin as a prebiotic source has also increased by 25.24%. Raw Berlin banana flour (UBF) contains resistant starch that has the potential to serve as a local alternative to inulin. This study aimed to investigate the effects of storage duration and prebiotic sources on the nutritional composition of synbiotic yogurt. A 2 × 3 factorial design was employed, with prebiotic type (Inulin and UBF) and storage time (days 0, 7, and 14) as factors. Each treatment was replicated twice with duplicate analyses. Proximate analyses included moisture, ash, carbohydrate, fat, protein, total energy, and fat energy contents. Data were analyzed using SPSS version 16 with independent t-tests and ANOVA, with significance set at p < 0.05. Results indicated that Storage time significantly affected the moisture, carbohydrate, fat, protein, total energy, and energy from fat contents (p < 0.05). The prebiotic sources inulin and UBF differed significantly in moisture and carbohydrate content on day 7, ash content on day 14, fat and energy from fat on days 0 and 7, and total energy on day 0. In conclusion, both storage duration and prebiotic source influenced the nutritional composition of the synbiotic yogurt.
Keywords : Local prebiotic source, proximate analysis, storage time, synbiotic yoghurt, unripe banana flour
- Abdalla, A. K., & Ahmed, Z. F. R. (2019). Physicochemical and sensory properties of yoghurt supplemented with green banana flour. Egyptian Journal of Dairy Science, 47(1), 1–9.
- Achmadin, N., Wardani, A., Sasmita, F., Hasanah, N., & Moentamaria, D. (2021). Studi kelayakan industri yoghurt. Distilat, 7(9), 310–319.
- Agustin, F., Damayati, R. P., & Febriyatna, A. (2024). Effect of the addition of unripe Berlin banana flour on the organoleptic characteristics of synbiotic yogurt. International Journal of Technology, Food and Agriculture, 1(2), 48–54. https://doi.org/10.25047/tefa.v1i2.4593
- Agustin, F., Putri, R., & Febriyatna, A. (2025). Effect of unripe Berlin banana flour on synbiotic yogurt: Physicochemical and microbiological properties. Jurnal Gizi dan Dietetik Indonesia, 13(1), 64–69.
- Amelia, F. Y., Warkoyo, W., Manshur, H. A., & Husna, A. (2022). Karakteristik organoleptik yoghurt sinbiotik dengan penambahan inulin pure pisang Barangan (Musa acuminata Colla). Food Technology and Halal Science Journal, 5(1), 32–44. https://doi.org/10.22219/fths.v5i1.18760
- Ayo-Omogie, H. N., Jolayemi, O. S., & Chinma, O. (2021). Fermentation and blanching as adaptable strategies to improve nutritional and functional properties of unripe Cardaba banana flour. Journal of Agriculture and Food Research, 6, 100214. https://doi.org/10.1016/j.jafr.2021.100214
- Burkhart, S., Underhill, S., & Raneri, J. (2022). Realizing the potential of neglected and underutilized bananas in improving diets for nutrition and health outcomes in the Pacific Islands. Frontiers in Sustainable Food Systems, 6(March). https://doi.org/10.3389/fsufs.2022.805776
- Chen, C., Zhao, S., Hao, G., Yu, H., Tian, H., & Zhao, G. (2017). Role of lactic acid bacteria on the yogurt flavour: A review. International Journal of Food Properties, 20(1), S316–S330. https://doi.org/10.1080/10942912.2017.1295988
- Dias, P. G. I., Sajiwani, J. W. A., & Rathnayaka, R. M. U. S. K. (2020). Consumer perception and sensory profile of probiotic yogurt with added sugar and reduced milk fat. Heliyon, 6(April), e04328. https://doi.org/10.1016/j.heliyon.2020.e04328
- Falah, F., Vasiee, A., & Yazdi, F. T. (2021). Preparation and functional properties of synbiotic yogurt fermented with Lactobacillus brevis PML1 derived from a fermented cereal-dairy product. 2021.
- Handayani, I., & Nuraini, N. (2022). Effect of concentration of Ambon banana flour and addition of Lactobacillus casei on chemical characteristics of synbiotic yogurt. Jurnal Agrotek Ummat, 1. https://journal.ummat.ac.id/index.php/agrotek/article/view/8452
- Kamel, D. G., Hammam, A. R. A., Alsaleem, K. A., & Osman, D. M. (2021). Addition of inulin to probiotic yogurt: Viability of probiotic bacteria (Bifidobacterium bifidum) and sensory characteristics. Food Science and Nutrition, 9(3), 1743–1749. https://doi.org/10.1002/fsn3.2154
- Li, S., Tang, S., He, Q., Hu, J., & Zheng, J. (2019). Changes in proteolysis in fermented milk produced by Streptococcus thermophilus in co-culture with Lactobacillus plantarum or Bifidobacterium animalis. Molecules, 24(20), 3699. https://doi.org/10.3390/molecules24203699
- Majdiyyah, M., & Farida, E. (2023). Pengaruh masa simpan terhadap karakteristik fisik dan mikrobiologi yoghurt sinbiotik ubi jalar kuning (Ipomoea batatas L.). Indonesian Journal of Public Health and Nutrition, 3(1), 80–87. https://doi.org/10.15294/ijphn.v3i1.60012
- Marini, T., Aparecida, D., Harumi, E., Nunes, A., Anaya, K., Elisabete, A., Antunes, C., Teresa, M., & Pacheco, B. (2022). Development of probiotic yoghurts with high protein content by ultrafiltration. NFS Journal, 29(October), 16–25. https://doi.org/10.1016/j.nfs.2022.09.003
- Munir, H., Alam, H., Nadeem, M. T., Almalki, R. S., Arshad, M. S., & Suleria, H. A. R. (2024). Green banana resistant starch: A promising potential as functional ingredient against certain maladies. Food Science and Nutrition, 12(6), 3787–3805. https://doi.org/10.1002/fsn3.4063
- Pangestu, A. D., Kuriniawan., & Supriyadi. (2021). Pengaruh variasi suhu dan lama penyimpanan terhadap viabilitas bakteri asam laktat (BAL) dan nilai pH yogurt. Borneo Journal of Medical Laboratory Technology, 3(2), 231–236.
- Roy, S., & Dhaneshwar, S. (2023). Role of prebiotics, probiotics, and synbiotics in management of inflammatory bowel disease: Current perspectives. World Journal of Gastroenterology, 29(14), 2078–2100. https://doi.org/10.3748/WJG.V29.I14.2078
- Saleh, A., Mohamed, A. A., Alamri, M. S., Hussain, S., Qasem, A. A., & Ibraheem, M. A. (2020). Effect of different starches on the rheological, sensory and storage attributes of non-fat set yogurt. Foods, 9(1). https://doi.org/10.3390/foods9010061
- Sanett Matela, K., Karuppiah Pillai, M., Matebesi-Ranthimo, P., & Ntakatsane, M. (2019). Analysis of proximate compositions and physiochemical properties of some yoghurt samples from Maseru, Lesotho. Journal of Food Science and Nutrition Research, 2(3), 245–252. https://doi.org/10.26502/jfsnr.2642-11000023
- Setiadi, O. Y., Sumarmono, J., & Setyawardani, T. (2023). Bulletin of applied animal research. Bulletin of Applied Animal Research, 5(September), 6–18.
- Sumi, K., Tagawa, R., Yamazaki, K., Nakayama, K., Ichimura, T., Sanbongi, C., & Nakazato, K. (2023). Nutritional value of yogurt as a protein source: Digestibility/absorbability and effects on skeletal muscle. Nutrients, 15(20), 1–16. https://doi.org/10.3390/nu15204366
- Zbikowska, A., Szymańska, I., & Kowalska, M. (2020). Impact of inulin addition on properties of natural yogurt. Applied Sciences, 10(12). https://doi.org/10.3390/app10124317
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