Supp. File(s): Copyright Agreement
Asupan vitamin A, vitamin C, vitamin E, BCAA dan kaitannya dengan kadar hs-CRP pada member fitness center dewasa
Excessive physical exercise can stimulate proinflammatory cytokines expressions and increases high sensitivity C-Reactive Protein (hs-CRP). Branched Chain Amino Acids (BCAA) and vitamins A, C, and E are antioxidant mechanisms to reduce oxidative stress. This study analyzes the correlation between vitamin A, C, E, and BCAA intake with hs-CRP. The study design was cross-sectional, which was conducted in Semarang. Participants were 48 men aged 20-43 years. Sampling used consecutive sampling. Data were collected using food recall 3x24h to assess dietary intake and laboratory tests using the Immunoturbidimetric method to determine hs-CRP levels. Data were statistically analyzed using Spearman-Rank and multiple linear regression. The result showed that the subject with high-risk categories of hs-CRP was 18,8%. The intake of BCAA was 10,8% from supplements. Most subjects had an excessive intake of vitamin A but did not meet the needs of vitamins C and E. There was no correlation between BCAA intake (p= 0,681) and vitamin C intake (p= 0,137) with hs-CRP levels. However, there was a negative correlation between vitamin A (p= 0,003) and vitamin E (p= 0,013) intake with hs-CRP levels. Multivariate analysis showed that the intake of vitamin A associated with hs-CRP levels of 8,1% (p= 0,028). In conclusion, vitamin A intake is the most influential factor in hs-CRP levels in fitness center members.
Supplement Files
Keywords : Amino acids, Reactive protein, Vitamin intake
- Angeli, F., Reboldi, G., & Verdecchia, P. (2021). The link between inflammation and hypertension: Unmasking mediators. American Journal of Hypertension, 34(7), 683–685. https://doi.org/10.1093/ajh/hpab034
- Apriantono, T., Hasan, F., Bahri, S., Bahana, I., & Rahadian, R. A. (2021). Level of nutrition knowledge personal trainer in West java. Jurnal SPORTIF: Jurnal Penelitian Pembelajaran, 7(3), 454–466. https://doi.org/10.29407/js_unpgri.v7i3.16812
- Borel, P., Preveraud, D., & Desmarchelier, C. (2013). Bioavailability of vitamin E in humans: an update. Nutrition Reviews, 71(6), 319–331. https://doi.org/10.1111/nure.12026
- Cadegiani, F. A., & Kater, C. E. (2019). Basal hormones and biochemical markers as predictors of overtraining syndrome in male athletes: The EROS-Basal study. Journal of Athletic Training, 54(8), 906–914. https://doi.org/10.4085/1062-6050-148-18
- Campa, F., Gobbo, L. A., Stagi, S., Cyrino, L. T., Toselli, S., Marini, E., & Coratella, G. (2022). Bioelectrical impedance analysis versus reference methods in the assessment of body composition in athletes. European Journal of Applied Physiology, 122(3), 561–589. https://doi.org/10.1007/s00421-021-04879-y
- Cheng, A. J., Jude, B., & Lanner, J. T. (2020). Intramuscular mechanisms of overtraining. Redox Biology, 35. https://doi.org/10.1016/j.redox.2020.101480
- de Souza, D. C., dos Santos, J. A. B., de Jesus, D. M., & Gentil, P. (2018). Biochemical profile and body composition alteration of amateur bodybuilders during the pre-contest period. Journal of Functional Morphology and Kinesiology, 3(2), 1–10. https://doi.org/10.3390/jfmk3020026
- Dias, T. R., Martin-Hidalgo, D., M. Silva, B., F. Oliveira, P., & G. Alves, M. (2020). endogenous and exogenous antioxidants as a tool to ameliorate male infertility induced by reactive oxygen species. Antioxidants and Redox Signaling, 33(11), 767–785. https://doi.org/10.1089/ars.2019.7977
- Farajbakhsh, A., Mazloomi, S. M., Mazidi, M., Rezaie, P., Akbarzadeh, M., Ahmad, S. P., Ferns, G. A., Ofori-Asenso, R., & Babajafari, S. (2019). Sesame oil and vitamin E co-administration may improve cardiometabolic risk factors in patients with metabolic syndrome: a randomized clinical trial. European Journal of Clinical Nutrition, 73(10), 1403–1411. https://doi.org/10.1038/s41430-019-0438-5
- Flory, S., Birringer, M., & Frank, J. (2019). Bioavailability and metabolism of vitamin E. In Vitamin E in Human Health (pp. 31–41). Springer International Publishing. https://doi.org/10.1007/978-3-030-05315-4_4
- Fouré, A., & Bendahan, D. (2017). Is branched-chain amino acids supplementation an efficient nutritional strategy to alleviate skeletal muscle damage? A systematic review. Nutrients, 9(10), 1–15. https://doi.org/10.3390/nu9101047
- Giri, M. K. W. (2019). Immunological side in overtraining exercise. International Journal of Health & Medical Sciences, 2(1), 1–6. https://doi.org/10.31295/ijhms.v2n1.52
- Gropper, S. S., Smith, J. L., & Carr, T. P. (2018). Advanced Nutrition and Human Metabolism 7th edition. Cengage Learning.
- Hajizadeh-Sharafabad, F., Zahabi, E. S., Malekahmadi, M., Zarrin, R., & Alizadeh, M. (2021). Carotenoids supplementation and inflammation: a systematic review and meta-analysis of randomized clinical trials. Critical Reviews in Food Science and Nutrition, 1–17. https://doi.org/10.1080/10408398.2021.1925870
- Halson, S. L. (2014). Monitoring training load to understand fatigue in athletes. Sports Medicine, 44(suppl 2), 139–147. https://doi.org/10.1007/s40279-014-0253-z
- Hamaya, R., Mora, S., Lawler, P. R., Cook, N. R., Ridker, P. M., Buring, J. E., Lee, I. M., Manson, J. A. E., & Tobias, D. K. (2021). Association of plasma branched-chain amino acid with biomarkers of inflammation and lipid metabolism in women. Circulation: Genomic and Precision Medicine, 14(4), E003330. https://doi.org/10.1161/CIRCGEN.121.003330
- He, F., Chuang, C. C., Zhou, T., Jiang, Q., Sedlock, D. A., & Zuo, L. (2018). Redox correlation in muscle lengthening and immune response in eccentric exercise. PLoS ONE, 13(12). https://doi.org/10.1371/journal.pone.0208799
- Higgins, M. R., Izadi, A., & Kaviani, M. (2020). Antioxidants and exercise performance: with a focus on vitamin e and c supplementation. International Journal of Environmental Research and Public Health, 17(22), 1–26. https://doi.org/10.3390/ijerph17228452
- Howatson, G., Hoad, M., Goodall, S., Tallent, J., Bell, P. G., & French, D. N. (2012). Exercise-induced muscle damage is reduced in resistance-trained males by branched chain amino acids: a randomized, double-blind, placebo controlled study. Journal of the International Society of Sports Nutrition, 9(20), 1–7. http://www.jissn.com/content/9/1/20
- Jackman, S. R., Witard, O. C., Jeukendrup, A. E., & Tipton, K. D. (2010). Branched-chain amino acid ingestion can ameliorate soreness from eccentric exercise. Medicine & Science in Sports & Exercise, 42(5), 962–970. https://doi.org/10.1249/MSS.0b013e3181c1b798
- Jäger, R., Kerksick, C. M., Campbell, B. I., Cribb, P. J., Wells, S. D., Skwiat, T. M., & et al. (2017). International society of sports nutrition position stand: Protein and exercise. Journal of the International Society of Sports Nutrition, 14(1), 1–25. https://doi.org/10.1186/s12970-017-0177-8
- Jahangiri, Z., Gholamnezhad, Z., Hosseini, M., Beheshti, F., & Kasraie, N. (2019). The effects of moderate exercise and overtraining on learning and memory, hippocampal inflammatory cytokine levels, and brain oxidative stress markers in rats. Journal of Physiological Sciences, 69(6), 993–1004. https://doi.org/10.1007/s12576-019-00719-z
- Jiang, Q. (2022). Metabolism of natural forms of vitamin E and biological actions of vitamin E metabolites. Free Radical Biology and Medicine, 179, 375–387. https://doi.org/10.1016/j.freeradbiomed.2021.11.012
- Kementerian Kesehatan Republik Indonesia. (2019). Angka kecukupan gizi yang dianjurkan untuk masyarakat Indonesia. kementrian keseharan RI.
- Kerksick, C. M., & Kulovitz, M. (2013). Requirements of Energy, Carbohydrates, Proteins and Fats for Athletes. Nutrition and Enhanced Sports Performance, 355–366. https://doi.org/10.1016/B978-0-12-396454-0.00036-9
- Lykkesfeldt, J. (2020). On the effect of vitamin C intake on human health: How to (mis)interprete the clinical evidence. Redox Biology, 34, 101532. https://doi.org/10.1016/j.redox.2020.101532
- MacInnis, M. J., & Gibala, M. J. (2017). Physiological adaptations to interval training and the role of exercise intensity. Journal of Physiology, 595(9), 2915–2930. https://doi.org/10.1113/JP273196
- Magherini, F., Fiaschi, T., Marzocchini, R., Mannelli, M., Gamberi, T., Modesti, P. A., & Modesti, A. (2019). Oxidative stress in exercise training: the involvement of inflammation and peripheral signals. Free Radical Research, 53(11–12), 1155–1165. https://doi.org/10.1080/10715762.2019.1697438
- Mazurek, K., Zmijewski, P., Czajkowska, A., & Lutosławska, G. (2011). High-sensitivity c-reantive protein (hsCRP) in young adults relation to aerobic capacity physical activity and risk factors for cardiovascular disease. Biology of Sport, 28(4), 227–232. https://doi.org/10.5604/965482
- McKean, M., Mitchell, L., O’Connor, H., Prvan, T., & Slater, G. (2019). Are exercise professionals fit to provide nutrition advice? An evaluation of general nutrition knowledge. Journal of Science and Medicine in Sport, 22(3), 264–268. https://doi.org/10.1016/j.jsams.2018.08.018
- Michaud, M., Balardy, L., Moulis, G., Gaudin, C., Peyrot, C., Vellas, B., Cesari, M., & Nourhashemi, F. (2013). Proinflammatory cytokines, aging, and age-related diseases. Journal of the American Medical Directors Association, 14(12), 877–882. https://doi.org/10.1016/j.jamda.2013.05.009
- Miyazawa, T., Burdeos, G. C., Itaya, M., Nakagawa, K., & Miyazawa, T. (2019). Vitamin E: Regulatory redox interactions. IUBMB Life, 71(4), 430–441. https://doi.org/10.1002/iub.2008
- Moutachakkir, M., Hanchi, A. L., Baraou, A., Boukhira, A., & Chellak, S. (2017). Caractéristiques immunoanalytiques de la protéine C-réactive et de la protéine C-réactive ultrasensible. Annales de Biologie Clinique, 75(2), 225–229. https://doi.org/10.1684/abc.2017.1232
- Negida, A. (2020). Sample size calculation guide-Part 7: How to calculate the sample size based on a correlation. Advanced Journal of Emergency Medicine, 4(2), e34. https://doi.org/10.22114/ajem.v0i0.344
- Niknam, M., & Paknahad, Z. (2015). Anti-inflammatory effects of dietary antioxidants in patients with coronary artery disease. Endocrinology & Metabolic Syndrome, 4(4). https://doi.org/10.4172/2161-1017.1000207
- Pahwa, R., Goyal, A., & Jialal, I. (2022). Chronic inflammation. StatPearls Publishing.
- Pérez-gálvez, A., Viera, I., & Roca, M. (2020). Carotenoids and chlorophylls as antioxidants. Antioxidants, 9(6), 1–39. https://doi.org/10.3390/antiox9060505
- Plotkin, D. L., Delcastillo, K., van Every, D. W., Tipton, K. D., Aragon, A. A., & Schoenfeld, B. J. (2021). Isolated leucine and branched-chain amino acid supplementation for enhancing muscular strength and hypertrophy: A narrative review. International Journal of Sport Nutrition and Exercise Metabolism, 31(3), 292–301. https://doi.org/10.1123/IJSNEM.2020-0356
- Putri, M. A. (2018). Peningkatan Antioksidan endogen yang dipicu latihan fisik increased endogenous antioxidants triggered by physical exercise. Jurnal Kedokteran YARSI, 26(3), 163–172. https://academicjournal.yarsi.ac.id/index.php/jurnal-fk-yarsi/article/view/760
- Puzianowska-Kuźnicka, M., Owczarz, M., Wieczorowska-Tobis, K., Nadrowski, P., Chudek, J., Slusarczyk, P., Skalska, A., Jonas, M., Franek, E., & Mossakowska, M. (2016). Interleukin-6 and C-reactive protein, successful aging, and mortality: The PolSenior study. Immunity and Ageing, 13(1), 1–12. https://doi.org/10.1186/s12979-016-0076-x
- Righi, N. C., Schuch, F. B., de Nardi, A. T., Pippi, C. M., de Almeida Righi, G., Puntel, G. O., da Silva, A. M. V., & Signori, L. U. (2020). Effects of vitamin C on oxidative stress, inflammation, muscle soreness, and strength following acute exercise: meta-analyses of randomized clinical trials. European Journal of Nutrition, 59(7), 2827–2839. https://doi.org/10.1007/s00394-020-02215-2
- Rusiani, E., Junaidi, S., Subiyono, H. S., & Sumartiningsih, S. (2019). Suplementasi vitamin C dan E untuk menurunkan stres oksidatif setelah melakukan aktivitas fisik maksimal. Media Ilmu Keolahragaan Indonesia, 9(2). https://journal.unnes.ac.id/nju/index.php/miki/article/view/23582
- Safabakhsh, M., Emami, M. R., Zeinali Khosroshahi, M., Asbaghi, O., Khodayari, S., Khorshidi, M., Alizadeh, S., & Viri, E. H. (2020). Vitamin C supplementation and C-reactive protein levels: Findings from a systematic review and meta-analysis of clinical trials. Journal of Complementary and Integrative Medicine. https://doi.org/10.1515/jcim-2019-0151
- Sari, T. (2013). Faktor-faktor yang berhubungan dengan konsumsi suplemen asam amino pada anggota Fitness Center Syahida Inn UIN Syarif Hidayatullah Jakarta tahun 2013 (Skripsi). UIN Syarif Hidayatullah Jakarta.
- Sarin, H. v., Lee, J. H., Jauhiainen, M., Joensuu, A., Borodulin, K., Männistö, S., Jin, Z., Terwilliger, J. D., Isola, V., Ahtiainen, J. P., Häkkinen, K., Kristiansson, K., Hulmi, J. J., & Perola, M. (2019). Substantial fat mass loss reduces low-grade inflammation and induces positive alteration in cardiometabolic factors in normal-weight individuals. Scientific Reports, 9(1), 1–14. https://doi.org/10.1038/s41598-019-40107-6
- Schneider, C. D., Bock, P. M., Becker, G. F., Moreira, J. C. F., Bello-Klein, A., & Oliveira, A. R. (2018). Comparison of the effects of two antioxidant diets on oxidative stress markers in triathletes. Biology of Sport, 35(2), 181–189. https://doi.org/10.5114/biolsport.2018.74194
- Schwab, S., Zierer, A., Schneider, A., Heier, M., Koenig, W., Kastenmüller, G., Waldenberger, M., Peters, A., & Thorand, B. (2015). Vitamin E supplementation is associated with lower levels of C-reactive protein only in higher dosages and combined with other antioxidants: The Cooperative Health Research in the Region of Augsburg (KORA) F4 study. British Journal of Nutrition, 113(11), 1782–1791. https://doi.org/10.1017/S0007114515000902
- Shenoy, S., Dhawan, M., & Sandhu, J. S. (2017). Effect of chronic supplementation of branched chain amino acids on exercise-induced muscle damage in trained athletes. Journal of Sports Science, 5, 265–273. https://doi.org/10.17265/2332-7839/2017.05.005
- Shimomura, Y., Inaguma, A., Watanabe, S., Yamamoto, Y., Muramatsu, Y., Bajotto, G., Sato, J., Shimomura, N., Kobayashi, H., & Mawatari, K. (2010). Branched-chain amino acid supplementation before squat exercise and delayed-onset muscle soreness. International Journal of Sport Nutrition and Exercise Metabolism, 20(3), 236–244. https://doi.org/10.1123/ijsnem.20.3.236
- Siahkouhian, M., & Esmaeilzadeh, S. (2011). Comparisons of serum C-reactive protein in young soccer players and non-athletes. Biology of Sport, 28(4), 255–258.
- Sinha, S. K., Nicholas, S. B., Sung, J. H., Correa, A., Rajavashisth, T. B., Norris, K. C., & Lee, J. E. (2019). Hs-CRP is associated with incident diabetic nephropathy: Findings from the jackson heart study. Diabetes Care, 42(11), 2083–2089. https://doi.org/10.2337/dc18-2563
- Suharjana, S. (2013). Analisis program kebugaran jasmani pada pusat-pusat kebugaran jasmani di Yogayakarta. Medikora, XI(2), 135–149.
- William, M. (2010). Body weight and composition for health and sport. In: Nutrition for Health, Fitness, and Sport. 9th editio. Graw-Hill.
- Williams, K., Carrasquilla, G. D., Ingerslev, L. R., Hochreuter, M. Y., Hansson, S., Pillon, N. J., Donkin, I., Versteyhe, S., Zierath, J. R., Kilpeläinen, T. O., & Barrès, R. (2021). Epigenetic rewiring of skeletal muscle enhancers after exercise training supports a role in whole-body function and human health. Molecular Metabolism, 53, 1–13. https://doi.org/10.1016/j.molmet.2021.101290
- Wolfe, R. R. (2017). Branched-chain amino acids and muscle protein synthesis in humans: Myth or reality? Journal of the International Society of Sports Nutrition, 14(1), 1–7. https://doi.org/10.1186/s12970-017-0184-9


This work is licensed under a Creative Commons Attribution-ShareAlike 4.0 International License.