Investigation of antioxidant activity from beetroot juice (Beta vulgaris L) as a healthy drink for the prevention of non-communicable disease

Ratih Tri Amelia* -  1 Program Studi Magister Ilmu Gizi, Universitas Sebelas Maret, Indonesia
Tonang Dwi Ardyanto -  Departemen Pendidikan dan Penelitian, Rumah Sakit Universitas Sebelas Maret, Indonesia
Yulia Sari -  Departemen Parasitologi Fakultas Kedokteran, Universitas Sebelas Maret, Indonesia

Supp. File(s): Research Results Ethical Clearance Copyright Agreement Covering Letters Etika Publikasi

Non-communicable diseases can cause oxidative stress, which affects their pathophysiology. One of the recommended diets for preventing complications of non-communicable diseases (NCD) is a rich antioxidant diet. One of the foods rich in antioxidants is beetroots. This study aimed to assess the antioxidant content of locally obtained beetroot juice. Laboratory experimental methods were used to test the antioxidant activity of beetroot juice. Before the laboratory test, the panelists carried out a hedonic test. The beets were then juiced and checked for DPPH′ (2,2-diphenyl-1-picrylhydrazyl). The beetroot was then juiced and checked for DPPH′ (2,2-diphenyl-1-picrylhydrazyl). Statistical analysis was performed using the Kruskal–Wallis test.  Results: The hedonic test showed that panelists preferred beetroot juice with lemon juice, with a 'like' range for the aroma and viscosity and a 'neutral-like' range for the taste and color of beetroot juice. The antioxidant content in 300 ml of beetroot juice was 53,34 ± 0,13%. In conclusion, beetroot juice has a high antioxidant content and health benefits in preventing NCD.

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Keywords : Acceptance test, antioxidant, beetroot, diet, health product

  1. Abedimanesh, N., Asghari, S., Mohammadnejad, K., Daneshvar, Z., Rahmani, S., Shokoohi, S., Farzaneh, A. H., Hosseini, S. H., Jafari Anarkooli, I., Noubarani, M., Andalib, S., Eskandari, M. R., & Motlagh, B. (2021). The anti-diabetic effects of betanin in streptozotocin-induced diabetic rats through modulating AMPK/SIRT1/NF-κB signaling pathway. Nutrition & Metabolism, 18(1), 92. https://doi.org/10.1186/s12986-021-00621-9
  2. Adnan, M. L., Pramaningtyas, M. D., Islamiana, D., & Sudarto, H. A. (2022). Hyperlipidemia diet reduces superoxide dismutase inhibition rate in the brain organ of rattus norvegicus. Mutiara Medika: Jurnal Kedokteran Dan Kesehatan, 22(1), 14–19. https://doi.org/10.18196/mmjkk.v22i1.8167
  3. Afshin, A., Micha, R., Khatibzadeh, S., Schmidt, L. A., & Mozaffarian, D. (2014). Dietary policies to reduce non‐communicable diseases. In The Handbook of Global Health Policy (pp. 175–193). Wiley. https://doi.org/10.1002/9781118509623.ch9
  4. Ahmad, A., Dempsey, S. K., Daneva, Z., Azam, M., Li, N., Li, P. L., & Ritter, J. K. (2018). Role of nitric oxide in the cardiovascular and renal systems. International Journal of Molecular Sciences, 19(9). https://doi.org/10.3390/ijms19092605
  5. Al-Hadlaq, S. M., Balto, H. A., Hassan, W. M., Marraiki, N. A., & El-Ansary, A. K. (2022). Biomarkers of non-communicable chronic disease: an update on contemporary methods. PeerJ, 10, 1–43. https://doi.org/10.7717/peerj.12977
  6. Al Rahmad, A. H. (2021). Several risk factors of obesity among female school teachers and relevance to non-communicable diseases during the Covid-19 pandemic. Amerta Nutrition, 5(1), 31–40. http://dx.doi.org/10.20473/amnt.v5i1.2021.31-40
  7. Aliahmadi, M., Amiri, F., Bahrami, L. S., Hosseini, A. F., Abiri, B., & Vafa, M. (2021). Effects of raw red beetroot consumption on metabolic markers and cognitive function in type 2 diabetes patients. Journal of Diabetes and Metabolic Disorders, 20(1), 673–682. https://doi.org/10.1007/s40200-021-00798-z
  8. Allen, L., Williams, J., Townsend, N., Mikkelsen, B., Roberts, N., Foster, C., & Wickramasinghe, K. (2017). Socioeconomic status and non-communicable disease behavioural risk factors in low-income and lower-middle-income countries: a systematic review. The Lancet Global Health, 5(3), e277–e289. https://doi.org/10.1016/S2214-109X(17)30058-X
  9. Arazi, H., & Eghbali, E. (2021). Possible effects of beetroot supplementation on physical performance through metabolic, neuroendocrine, and antioxidant mechanisms: A Narrative Review of the Literature. Frontiers in Nutrition, 8(May), 1–19. https://doi.org/10.3389/fnut.2021.660150
  10. Ayoka, T. O., Ezema, B. O., Eze, C. N., & Nnadi, C. O. (2022). Antioxidants for the prevention and treatment of non-communicable diseases. Journal of Exploratory Research in Pharmacology, 000(000), 000–000. https://doi.org/10.14218/jerp.2022.00028
  11. Babarykin, D., Smirnova, G., Pundinsh, I., Vasiljeva, S., Krumina, G., & Agejchenko, V. (2019). Red beet (Beta vulgaris) impact on human health. Journal of Biosciences and Medicines, 07(03), 61–79. https://doi.org/10.4236/jbm.2019.73007
  12. Baião, D. dos S., da Silva, D. V. T., & Paschoalin, V. M. F. (2020). Beetroot, a remarkable vegetable: Its nitrate and phytochemical contents can be adjusted in novel formulations to benefit health and support cardiovascular disease therapies. Antioxidants, 9(10), 1–36. https://doi.org/10.3390/antiox9100960
  13. Baliyan, S., Mukherjee, R., Priyadarshini, A., Vibhuti, A., Gupta, A., Pandey, R. P., & Chang, C. M. (2022). Determination of antioxidants by DPPH radical scavenging activity and quantitative phytochemical analysis of ficus religiosa. Molecules, 27(4). https://doi.org/10.3390/molecules27041326
  14. Bruins, M. J., Van Dael, P., & Eggersdorfer, M. (2019). The role of nutrients in reducing the risk for noncommunicable diseases during aging. Nutrients, 11(1), 85. https://doi.org/10.3390/nu11010085
  15. Budreviciute, A., Damiati, S., Sabir, D. K., Onder, K., Schuller-Goetzburg, P., Plakys, G., Katileviciute, A., Khoja, S., & Kodzius, R. (2020). Management and prevention strategies for Non-communicable Diseases (NCDs) and their risk factors. Frontiers in Public Health, 8, 1–11. https://doi.org/https://doi.org/10.3389/fpubh.2020.574111
  16. Chen, L., Zhu, Y., Hu, Z., Wu, S., & Jin, C. (2021). Beetroot as a functional food with huge health benefits: Antioxidant, antitumor, physical function, and chronic metabolomics activity. Food Science and Nutrition, 9(11), 6406–6420. https://doi.org/10.1002/fsn3.2577
  17. Chhikara, N., Kushwaha, K., Sharma, P., Gat, Y., & Panghal, A. (2019). Bioactive compounds of beetroot and utilization in food processing industry: A critical review. Food Chemistry, 272, 192–200. https://doi.org/10.1016/j.foodchem.2018.08.022
  18. Dainy, N. C., & Yunieswati, W. (2023). Nutrition facts and antioxidant activity of spices biscuits as functional snacks to immune booster. AcTion: Aceh Nutrition Journal, 8(2), 165–175. https://doi.org/10.30867/action.v8i2.835
  19. Desseva, I., Stoyanova, M., Petkova, N., & Mihaylova, D. (2020). Red beetroot juice phytochemicals bioaccessibility: An in vitro approach. Polish Journal of Food and Nutrition Sciences, 70(1), 45–53. https://doi.org/10.31883/pjfns/116590
  20. Dhananjayan, I., Kathiroli, S., Subramani, S., & Veerasamy, V. (2017). Ameliorating effect of betanin, a natural chromoalkaloid by modulating hepatic carbohydrate metabolic enzyme activities and glycogen content in streptozotocin – nicotinamide induced experimental rats. Biomedicine and Pharmacotherapy, 88, 1069–1079. https://doi.org/10.1016/j.biopha.2017.01.146
  21. Esatbeyoglu, T., Wagner, A. E., Schini-Kerth, V. B., & Rimbach, G. (2015). Betanin-A food colorant with biological activity. Molecular Nutrition and Food Research, 59(1), 36–47. https://doi.org/10.1002/mnfr.201400484
  22. Finlay, B. B. (2020). Are noncommunicable diseases communicable? Science, 367(6475), 250–251. https://doi.org/10.1126/science.aaz3834
  23. Firdausia, R. S., Sholehah, K., Kurniasih, I., Diani, A., & Rusmeilina, R. (2023). Analisis potensi antioksidan daun kayu bulan (Pisonia alba Span.) sebagai Agen anti penuaan dini. Chimica et Natura Acta, 11(1), 22–28. https://doi.org/10.24198/cna.v11.n1.43034
  24. Fu, Y., Shi, J., Xie, S. Y., Zhang, T. Y., Soladoye, O. P., & Aluko, R. E. (2020). Red beetroot betalains: perspectives on extraction, processing, and potential health benefits. Journal of Agricultural and Food Chemistry, 68(42), 11595–11611. https://doi.org/10.1021/acs.jafc.0c04241
  25. Gantenbein, K. V., & Kanaka-Gantenbein, C. (2021). Mediterranean diet as an antioxidant: The impact on metabolic health and overall wellbeing. Nutrients, 13(6). https://doi.org/10.3390/nu13061951
  26. Gilchrist, M., Winyard, P. G., Aizawa, K., Anning, C., Shore, A., & Benjamin, N. (2013). Effect of dietary nitrate on blood pressure, endothelial function, and insulin sensitivity in type 2 diabetes. Free Radical Biology and Medicine, 60, 89–97. https://doi.org/10.1016/j.freeradbiomed.2013.01.024
  27. Guldiken, B., Toydemir, G., Nur Memis, K., Okur, S., Boyacioglu, D., & Capanoglu, E. (2016). Home-processed red beetroot (Beta vulgaris L.) products: Changes in antioxidant properties and bioaccessibility. International Journal of Molecular Sciences, 17(6). https://doi.org/10.3390/ijms17060858
  28. Karimzadeh, L., Sohrab, G., Hedayati, M., Ebrahimof, S., Emami, G., & Razavion, T. (2022). Effects of concentrated beetroot juice consumption on glycemic control, blood pressure, and lipid profile in type 2 diabetes patients: randomized clinical trial study. Irish Journal of Medical Science (1971 -), 0123456789. https://doi.org/10.1007/s11845-022-03090-y
  29. Liliana, C., & Oana-Viorela, N. (2020). Red Beetroot: Composition and health effects - a review. Journal of Nutritional Medicine and Diet Care, 5(2). https://doi.org/10.23937/2572-3278.1510043
  30. Luna, F., & Luyckx, V. A. (2020). Why have Non-communicable Diseases been Left Behind? Asian Bioethics Review, 12(1), 5–25. https://doi.org/10.1007/s41649-020-00112-8
  31. Martinez, R., Lloyd-Sherlock, P., Soliz, P., Ebrahim, S., Vega, E., Ordunez, P., & McKee, M. (2020). Trends in premature avertable mortality from non-communicable diseases for 195 countries and territories, 1990–2017: a population-based study. The Lancet Global Health, 8(4), e511–e523. https://doi.org/10.1016/S2214-109X(20)30035-8
  32. Novatama, S. M., Kusumo, E., & Supartono. (2016). Identifikasi betasianin dan uji antioksidan ekstrak buah bit merah (Beta vulgaris L). Indonesian Journal of Chemical Science, 5(3). https://doi.org/10.5962/bhl.title.81730
  33. Ocampo, D. A. B., Paipilla, A. F., Marín, E., Vargas-Molina, S., Petro, J. L., & Pérez-Idárraga, A. (2018). Dietary nitrate from beetroot juice for hypertension: A systematic review. Biomolecules, 8(4), 1–12. https://doi.org/10.3390/biom8040134
  34. Permanasari, D., Sari, A. E., & Aslam, M. (2021). Pengaruh konsentrasi gula terhadap aktivitas antioksidan pada minuman bir pletok. AcTion: Aceh Nutrition Journal, 6(1), 9. https://doi.org/10.30867/action.v6i1.321
  35. Pinheiro, L. C., Tanus-Santos, J. E., & Castro, M. M. (2017). The potential of stimulating nitric oxide formation in the treatment of hypertension. Expert Opinion on Therapeutic Targets, 21(5), 543–556. https://doi.org/10.1080/14728222.2017.1310840
  36. Purnamasari, D. (2018). The emergence of non-communicable disease in Indonesia. Acta Medica Indonesiana, 50(4), 273–274.
  37. Rahayu, M. S., Nurfardian, N., & Sari, A. N. (2023). The effectiveness of Jamblang (Syzygium cumini (L) Skeels) extract on the atherogenic index in a diabetic rat model. AcTion: Aceh Nutrition Journal, 8(4), 510–517. https://doi.org/10.30867/action.v8i4.1151
  38. Saxena, T., Ali, A. O., & Saxena, M. (2018). Pathophysiology of essential hypertension: an update. Expert Review of Cardiovascular Therapy, 16(12), 879–887. https://doi.org/10.1080/14779072.2018.1540301
  39. Senapati, S., Bharti, N., & Bhattacharya, A. (2015). Modern lifestyle diseases : chronic diseases, Awareness and Prevention. Int J Curr Res Acad Rev, 3(7), 215–223.
  40. Seyedsadjadi, N., & Grant, R. (2021). The potential benefit of monitoring oxidative stress and inflammation in the prevention of non-communicable diseases (NCDs). Antioxidants, 10(1), 1–32. https://doi.org/10.3390/antiox10010015
  41. Silva, D. V. T. da, Baião, D. dos S., Ferreira, V. F., & Paschoalin, V. M. F. (2021). Betanin as a multipath oxidative stress and inflammation modulator: a beetroot pigment with protective effects on cardiovascular disease pathogenesis. Critical Reviews in Food Science and Nutrition, 62(2), 539–554. https://doi.org/10.1080/10408398.2020.1822277
  42. Wruss, J., Waldenberger, G., Huemer, S., Uygun, P., Lanzerstorfer, P., Müller, U., Höglinger, O., & Weghuber, J. (2015). Compositional characteristics of commercial beetroot products and beetroot juice prepared from seven beetroot varieties grown in Upper Austria. Journal of Food Composition and Analysis, 42, 46–55. https://doi.org/10.1016/j.jfca.2015.03.005
  43. Zamani, H., de Joode, M. E. J. R., Hossein, I. J., Henckens, N. F. T., Guggeis, M. A., Berends, J. E., de Kok, T. M. C. M., & van Breda, S. G. J. (2021). The benefits and risks of beetroot juice consumption: a systematic review. Critical Reviews in Food Science and Nutrition, 61(5), 788–804. https://doi.org/10.1080/10408398.2020.1746629

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AcTion: Aceh Nutrition Journal
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