Changes in hemoglobin levels in relation to self-efficacy and adherence to weekly iron tablet supplementation among female high school students: a pre-experimental study

Mahfuzhoh Fadillah Heryanda* -  Bachelor of Science in Nutrition Program, Faculty of Public Health, Sriwijaya University, South Sumatra, Indonesia
Faradina Aghadiati -  Bachelor of Science in Nutrition Program, Faculty of Public Health, Sriwijaya University, South Sumatra
Devieka Rhama Dhanny -  Bachelor of Science in Nutrition Program, Faculty of Public Health, Sriwijaya University, South Sumatra, Indonesia
Devi Ardila -  Bachelor of Science in Nutrition Program, Faculty of Public Health, Sriwijaya University, South Sumatra, Indonesia
Widya Lestari Nurpratama -  Bachelor's Program in Nutrition, Faculty of Health Sciences, Suherman Medical University, West Java, Indonesia

Iron deficiency anemia (IDA) is a major public health problem, particularly among adolescent girls. The effectiveness of iron (Fe) tablet supplementation programs largely depends on adherence to consumption, which is influenced by behavioral factors such as self-efficacy. Low adherence reduces the success of iron tablet supplementation. This study aimed to analyze the changes in hemoglobin levels associated with self-efficacy and adherence to iron tablet consumption among adolescent girls. This study employed a pre-experimental, one-group, pretest–posttest design without a control group. The intervention consisted of administering iron tablets (60 mg elemental iron and 0.25 mg folic acid) once a week for one month. The study population included female adolescents from the Aulia Cendekia Talang Jambe Integrated Islamic Senior High School in Palembang City. A total of 33 subjects were selected using purposive sampling based on the inclusion and exclusion criteria. The variables in this study included participant characteristics, self-efficacy, adherence to iron tablet consumption, and changes in hemoglobin levels. Wilcoxon and chi-square tests were used for the data analysis. The results showed that 70.4% of patients with high self-efficacy experienced an increase in hemoglobin levels; however, there was no statistically significant relationship between self-efficacy and changes in hemoglobin levels (p = 0.338). In contrast, adherence to iron tablet consumption was significantly associated with changes in hemoglobin levels (p = 0.021). Non-adherent subjects were six times more likely to experience stable or decreased hemoglobin levels than adherent subjects (PR = 6.0; 95% CI: 1.2–28.9). In conclusion, adherence to iron tablet consumption is a significant factor influencing hemoglobin levels among the subjects.

Keywords : Adherence, iron tablet consumption, adolescent girls, iron deficiency anemia, self-efficacy, hemoglobin level

  1. Abdel-Khalek, A. M. (2016). Introduction to the psychology of self-esteem. In F. Holloway (Ed.), Self-esteem: Perspectives, influences, and improvement strategies (pp. 1–23). Nova Science Publishers.
  2. Ainaya, N. A., Apriningsih, Wahyuningtyas, W., & Makkiyah, F. A. (2022). Faktor-faktor yang memengaruhi niat remaja putri dalam mengonsumsi tablet tambah darah (TTD) di Desa Sirnagalih, Kabupaten Bogor. Jurnal Penelitian Kesehatan Suara Forikes, 13(2), 365–371. https://doi.org/10.33846/sf13220
  3. Ambarsari, N. D., Herlina, N., Dewanti, L., & Ernawati. (2023). Correlation between compliance with iron tablet consumption and iron nutrition intake with pregnant women’s hemoglobine consumption. The Indonesian Journal of Public Health, 18(1), 72–81. https://doi.org/10.20473/ijph.v18i1.2023.72-81
  4. Annisa, F. N., & Nurmala, I. (2018). Influence perceived benefit and perceived self-efficacy with intention of adolescent girls in consuming Fe tablet. Indian Journal of Public Health Research & Development, 9(4), 326–329. https://doi.org/10.5958/0976-5506.2018.00307.8
  5. Apriningsih, Madanijah, S., Dwiriani, C. M., & Kolopaking, R. (2020). Determinant of highschool girl adolescent’s adherence to consume iron folic acid supplementation in Kota Depok. Journal of Nutritional Science and Vitaminology, 66(Supplement), S369–S375. https://doi.org/10.3177/jnsv.66.S369
  6. Badan Kebijakan Pembangunan Kesehatan. (2024). Survei Kesehatan Indonesia (SKI) 2023 dalam angka. Kementerian Kesehatan Republik Indonesia. https://repository.badankebijakan.kemkes.go.id/id/eprint/5539/
  7. Broers, V. J. V., De Breucker, C., Van den Broucke, S., & Luminet, O. (2017). A systematic review and meta-analysis of the effectiveness of nudging to increase fruit and vegetable choice. European Journal of Public Health, 27(5), 912–920. https://doi.org/10.1093/eurpub/ckx085
  8. Chalatha Doa, N. S., Mediastuti, F., & Revika, E. (2024). Relationship between self-efficacy and adherence to Fe tablet consumption among adolescent girls. Jurnal Manajemen Kesehatan Indonesia, 12(1), 70–78. https://doi.org/10.14710/jmki.12.1.2024.70-78
  9. Cia, A., Annisa, S. N., & Lion, H. F. (2021). Asupan zat besi dan prevalensi anemia pada remaja usia 16–18 tahun. Window of Health: Jurnal Kesehatan, 4(2), 144–150. https://doi.org/10.33096/woh.vi.248
  10. Dinas Kesehatan Provinsi Sumatera Selatan. (2021). Profil kesehatan Provinsi Sumatera Selatan tahun 2020. https://ppid-dinkes.sumselprov.go.id/unggah/10028745-ProfilDinkes2021Data2020.pdf
  11. El-Kholy, A. A., El Kholy, E. A., Al Abdulathim, M. A., Abdou, A. H., Karar, H. A. D., Bushara, M. A., Abdelaal, K., & Sayed, R. (2023). Prevalence and associated factors of anemia among pregnant women and the impact of clinical pharmacist counseling on their awareness level: A cross-sectional study. Saudi Pharmaceutical Journal, 31(8), Article 101699. https://doi.org/10.1016/j.jsps.2023.101699
  12. Ems, T., St Lucia, K., & Huecker, M. R. (2023). Biochemistry, iron absorption. In StatPearls. StatPearls Publishing. https://www.ncbi.nlm.nih.gov/books/NBK448204/
  13. Fauzianty, A., Situmorang, F. N. S., Sari, S. N., Sabiah, S., Hasibuan, V. R., & Febriyan, Y. (2025). Effectiveness of a family-centered empowerment-based digital pocketbook on self-efficacy and iron supplement adherence among pregnant women: A quasi-experimental study. AcTion: Aceh Nutrition Journal, 10(4), 1184-1193. http://dx.doi.org/10.30867/action.v10i4.3039
  14. Hidayat, R. (2023). Pengaruh uang saku terhadap perilaku konsumtif pada siswa. Journal of Business Education and Social, 4(1), 57–64. https://doi.org/10.33592/jbes.v4i1.4274
  15. Hisa, K., Haruna, M., Hikita, N., Sasagawa, E., Yonezawa, K., Suto, M., & Ota, E. (2019). Prevalence of and factors related to anemia among Japanese adult women: Secondary data analysis using health check-up database. Scientific Reports, 9, Article 17048. https://doi.org/10.1038/s41598-019-52798-y
  16. Joe, W., Rinju, Patel, N., Alambusha, R., Kulkarni, B., Yadav, K., & Sethi, V. (2022). Coverage of iron and folic acid supplementation in India: Progress under the Anemia Mukt Bharat strategy 2017–20. Health Policy and Planning, 37(5), 597–606. https://doi.org/10.1093/heapol/czac015
  17. Kementerian Kesehatan Republik Indonesia. (2018a). Hasil utama Riset Kesehatan Dasar (Riskesdas) 2018. Badan Penelitian dan Pengembangan Kesehatan.
  18. Kementerian Kesehatan Republik Indonesia. (2018b). Pedoman pencegahan dan penanggulangan anemia pada remaja putri dan wanita usia subur (WUS). https://ayosehat.kemkes.go.id/buku-pedoman-pencegahan-dan-penanggulangan-anemia-pada-remaja-putri-dan-wanita-usia-subur
  19. Kumalasari, D., Kameliawati, F., Mukhlis, H., & Kristanti, D. A. (2019). Pola menstruasi dengan kejadian anemia pada remaja. Wellness and Healthy Magazine, 1(2), 187–192. https://doi.org/10.30604/well.28122019
  20. Lismiana, H., & Indarjo, S. (2021). Pengetahuan dan persepsi remaja putri terhadap kepatuhan konsumsi tablet tambah darah. Indonesian Journal of Public Health and Nutrition, 1(1), 22–30. https://doi.org/10.15294/ijphn.v1i1.45146
  21. Low, M. S. Y., Speedy, J., Styles, C. E., De-Regil, L. M., & Pasricha, S.-R. (2016). Daily iron supplementation for improving anaemia, iron status and health in menstruating women. Cochrane Database of Systematic Reviews, 2016(4), Article CD009747. https://doi.org/10.1002/14651858.CD009747.pub2
  22. Nanda, E., Maryana, N., Fadilah, M., Fauzia, A. R., Nurapandi, A., & Marliany, H. (2025). Efficacy of iron tablet supplementation on hemoglobin levels in adolescents: A systematic review. Genius Journal, 6(1), 272–283. https://doi.org/10.56359/gj.v6i1.528
  23. Nguyen, M., & Tadi, P. (2023). Iron supplementation. In StatPearls. StatPearls Publishing. https://www.ncbi.nlm.nih.gov/books/NBK557376/
  24. Nutrition International. (2022). Weekly iron and folic acid supplementation (WIFAS) for prevention of anaemia in menstruating women and adolescent girls and reducing the risk of neural tube defect (NTD)-affected pregnancies [Application for inclusion in the WHO Model List of Essential Medicines].
  25. Pai, R. D., Chong, Y. S., Clemente-Chua, L. R., Irwinda, R., Huynh, T. N. K., Wibowo, N., Gamilla, M. C. Z., & Mahdy, Z. A. (2023). Prevention and management of iron deficiency/iron-deficiency anemia in women: An Asian expert consensus. Nutrients, 15(14), Article 3125. https://doi.org/10.3390/nu15143125
  26. Permatasari, T. (2017). Pengaruh program pemberian tablet tambah darah (TTD) terhadap perbaikan status besi pada remaja putri di Kota Bogor [Master’s thesis, Institut Pertanian Bogor]. IPB University Scientific Repository. https://repository.ipb.ac.id/handle/123456789/88769
  27. Permatasari, T., Briawan, D., & Madanijah, S. (2018). Efektivitas program suplementasi zat besi pada remaja putri di Kota Bogor. Media Kesehatan Masyarakat Indonesia, 14(1), 1–8. https://doi.org/10.30597/mkmi.v14i1.3705
  28. Putri, A. T. (2025). Hubungan pengetahuan, self-efficacy, dan faktor lainnya terhadap kepatuhan konsumsi tablet tambah darah pada remaja putri usia 15–18 tahun di SMK Walisongo 2 Kota Depok tahun 2025 [Undergraduate thesis, Universitas Indonesia].
  29. Rehman, T., Agrawal, R., Ahamed, F., Das, S., Mitra, S., Kumar, D., Sethy, C., Kanungo, S., Bhattacharya, D., & Pati, S. (2025). Optimal dose and duration of iron supplementation for treating iron deficiency anaemia in children and adolescents: A systematic review and meta-analysis. PLOS ONE, 20(2), Article e0319068. https://doi.org/10.1371/journal.pone.0319068
  30. Salim, L. A., Silitonga, H. T. H., Nurmala, I., Muthmainnah, M., Devi, Y. P., Salsabila, A. C., & Restuti, D. Y. (2025). The effect of self identity on increasing iron tablet adherence among high school adolescent girls through Health Belief Model as mediator variables. Journal of Multidisciplinary Healthcare, 18, 4173–4183. https://doi.org/10.2147/JMDH.S527641
  31. Sari, N., & Sari, C. P. (2022). Perbedaan kadar hemoglobin sebelum dan sesudah mengkonsumsi tablet Fe pada remaja putri di SMK Taruna Padang tahun 2022. Jurnal Ilmiah PANNMED, 17(3), 1–9.
  32. Sari, P., Herawati, D. M. D., Dhamayanti, M., & Hilmanto, D. (2022). Anemia among adolescent girls in West Java, Indonesia: Related factors and consequences on the quality of life. Nutrients, 14(18), Article 3777. https://doi.org/10.3390/nu14183777
  33. Sari, V. M., Rahmatika, S. D., & Tonasih. (2022). Supplementary blood tablets (TTD) in adolescent women (Rematry) to increase hemoglobin (Hb) levels. Jurnal Kebidanan Malahayati, 8(2), 413–419. https://doi.org/10.33024/jkm.v8i2.6512
  34. Sartika, W., Herlina, S., Qomariah, S., & Juwita, S. (2022). Pengaruh uang saku terhadap kejadian gizi lebih pada remaja di masa pandemi COVID-19. Journal of Healthcare Technology and Medicine, 8(1), 400–405.
  35. Setianingsih, D., Heryanda, M. F., & Khoiriyah, N. (2025). Faktor yang mempengaruhi anemia pada remaja putri. Jurnal Bidan Komunitas, 8(3), 112–135.
  36. Shah, S. P., Shah, P., Desai, S., Modi, D., Desai, G., & Arora, H. (2016). Effectiveness and feasibility of weekly iron and folic acid supplementation to adolescent girls and boys through peer educators at community level in the tribal area of Gujarat. Indian Journal of Community Medicine, 41(2), 158–161. https://doi.org/10.4103/0970-0218.173498
  37. Silitonga, H. T. H., Salim, L. A., Nurmala, I., & Wartiningsih, M. (2023). Compliance of iron supplementation and determinants among adolescent girls: A systematic review. Iranian Journal of Public Health, 52(1), 37–48. https://doi.org/10.18502/ijph.v52i1.11664
  38. Stewart-Knox, B. J., Rankin, A., Bunting, B. P., Frewer, L. J., Celis-Morales, C., Livingstone, K. M., Fischer, A. R. H., Poínhos, R., Kuznesof, S., Gibney, M., & Mathers, J. C. (2022). Self-efficacy, habit strength, health locus of control and response to the personalised nutrition Food4Me intervention study. British Food Journal, 124(1), 314–330. https://doi.org/10.1108/BFJ-03-2021-0221
  39. Traissac, P., Montenegro, R., El Ati, J., Ben Gharbia, H., & Bour, A. (2020). In a nutrition transition context in North Africa, is the co-occurrence of excess adiposity and iron deficiency or anemia independent, aggravating or protective? Current Developments in Nutrition, 4(Supplement 2), Article 287. https://doi.org/10.1093/cdn/nzaa043_138
  40. Verma, K., & Baniya, G. C. (2022). Prevalence, knowledge, and related factor of anemia among school-going adolescent girls in a remote area of western Rajasthan. Journal of Family Medicine and Primary Care, 11(4), 1474–1481. https://doi.org/10.4103/jfmpc.jfmpc_1372_21
  41. Wangaskar, S. A., Sahu, S. K., Majella, M. G., & Rajaa, S. (2021). Prevalence of anaemia and compliance to weekly iron-folic acid supplementation programme amongst adolescents in selected schools of urban Puducherry, India. Nigerian Postgraduate Medical Journal, 28(1), 44–50. https://doi.org/10.4103/npmj.npmj_336_20
  42. Wiafe, M. A., Ayenu, J., & Eli-Cophie, D. (2023). A review of the risk factors for iron deficiency anaemia among adolescents in developing countries. Anemia, 2023, Article 6406286. https://doi.org/10.1155/2023/6406286
  43. Wolf, B. M., Herrmann, M., & Brandstätter, V. (2018). Self-efficacy vs. action orientation: comparing and contrasting two determinants of goal setting and goal striving. Journal of Research in Personality, 73, 35-45. https://doi.org/10.1016/j.jrp.2017.11.001
  44. World Health Organization Regional Office for South-East Asia. (2021). Rapid assessment of the implementation of adolescent health programmes in countries of South-East Asia. https://www.who.int/publications/i/item/9789290228639
  45. Yalestyarini, E. A., Fibrinia, A. A., Saputro, H., & Fazrin, I. (2026). Association between iron tablet adherence and anemia among adolescent girls. Journal of Nursing Practice, 9(3), 652–660. https://doi.org/10.30994/jnp.v9i3.1151
  46. Yani, M., Azhari, A., Al Rahmad, A. H., Bastian, F., Ilzana, T. M., Rahmi, C. R., Andriaty, S. N., Chanda, A., & Salsabila, S. (2023). The relationship between menarche and nutritional status in Junior High School students in Aceh Besar. A study from 30 years of armed conflict area, Aceh, Indonesia. AcTion: Aceh Nutrition Journal, 8(4), 635–641. https://doi.org/10.30867/action.v8i4.1310

Open Access Copyright (c) 2026 Mahfuzhoh Fadillah Heryanda, Faradina Aghadiati, Devieka Rhama Dhanny, Devi Ardila, Widya Lestari Nurpratama
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AcTion: Aceh Nutrition Journal
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