Effect of yacon (Smallanthus sossnchifolius) tuber extract addition on chemical and organoleptic characteristics of green tea kombucha

Nabilah Hening Prayitno* -  Master of Nutrition Science, Department of Nutrition, Faculty of Health Sciences, Universitas Brawijaya, Malang, Indonesia
Irma Sarita Rahmawati -  Department of Nutrition, Faculty of Health Sciences, Universitas Brawijaya, Malang, Indonesia
Fajar Ari Nugroho -  Department of Nutrition, Faculty of Health Sciences, Universitas Brawijaya, Malang, Indonesia
DOI : 10.30867/action.v11i2.3093

Kombucha is a fermented beverage produced from tea, sugar, and a symbiotic culture of microorganisms. Yakon tubers have a natural sweetness and contain phenolic compounds, fructooligosaccharides, and inulin, which function as natural prebiotics. However, studies on the incorporation of yakon tubers into green tea kombucha are limited. This study aimed to evaluate the effect of yakon tuber extract on the chemical and organoleptic characteristics of green tea kombucha. A true experimental design with a Completely Randomized Design was applied using four formulations: F0 (0%), F1 (10%), F2 (20%), and F3 (30%). The parameters analyzed included pH, total sugar content (DNS method), total flavonoid content (UV–Vis spectrophotometry), and sensory evaluation by 30 panelists, carried out for 1 month at Universitas Brawijaya. Data were analyzed using one-way ANOVA/Kruskal–Wallis/Mann–Whitney and Duncan’s Multiple Range Test (DMRT), as appropriate. The results showed that the addition of yakon extract reduced the total sugar content (p=0.015) and increased the total flavonoid content (p=0.000). The final pH values were within a safe range (2.54–2.62), with no significant differences in the sensory attributes (p>0.05). Yakon tubers support microbial growth during fermentation. Formulation F3 (30%) was identified as the best treatment based on its acceptable pH, lower sugar content after dilution, and highest panelist preference.

Keywords : Kombucha, green tea, yacon tuber

  1. Cardoso, R. R., Neto, R. O., dos Santos D’Almeida, C. T., do Nascimento, T. P., Pressete, C. G., Azevedo, L., Martino, H. S. D., Cameron, L. C., Ferreira, M. S. L., & Barros, F. A. R. de. (2020). Kombuchas from green and black teas have different phenolic profiles, which impact antioxidant capacities, antibacterial and antiproliferative activities. Food Research International, 128, 108782. https://doi.org/10.1016/j.foodres.2019.108782
  2. Chessum, K., Chen, T., Kam, R., & Yan, M. (2023). A comprehensive chemical and nutritional analysis of New Zealand yacon concentrate. Foods, 12(1). https://doi.org/10.3390/foods12010074
  3. Cohen, G., Sela, D. A., & Nolden, A. A. (2023). Sucrose concentration and fermentation temperature impact the sensory characteristics and liking of kombucha. Foods, 12(16). https://doi.org/10.3390/foods12163116
  4. de Miranda, J. F., Ruiz, L. F., Silva, C. B., Uekane, T. M., Silva, K. A., Gonzalez, A. G. M., Fernandes, F. F., & Lima, A. R. (2022). Kombucha: A review of substrates, regulations, composition, and biological properties. Journal of Food Science, 87(2), 503–527. https://doi.org/10.1111/1750-3841.16029
  5. Dionísio, A. P., da Silva, M. de F. G., Carioca, A. A. F., Adriano, L. S., de Abreu, F. A. P., Wurlitzer, N. J., Pinto, C. de O., & Pontes, D. F. (2020). Effect of yacon syrup on blood lipid, glucose and metabolic endotoxemia in healthy subjects: A randomized, double-blind, placebo-controlled pilot trial. Food Science and Technology, 40(1), 194–201. https://doi.org/10.1590/fst.38218
  6. Durães, G. C. S., Pires, B. A. M., & Lins, T. C. L. (2021). Kombucha-based synbiotic beverage using yacon (Smallanthus sonchifolius) as a fermentation substrate: Development and sensorial analysis. Food Research, 5(4), 66–71. https://doi.org/10.26656/fr.2017.5(4).703
  7. Essiedu, J. A., Areerate, P., & Withayagiat, U. (2024). Evaluation of physiochemical composition, phenolic compounds, and antioxidant activity of kombucha produced from Thunbergia laurifolia as a potential functional food. International Journal of Food Science and Technology, 59(10), 6999–7010. https://doi.org/10.1111/ijfs.17408
  8. Fawwaz, M., Muflihunna, A., Pratama, M., Rahmawati, R., Razak, R., & Baits, M. (2022). Total phenolic and flavonoid compound of crude and purified extract of green tea leaves (Camellia sinensis) from Makassar, Indonesia. Jurnal Fitofarmaka Indonesia, 9(3), 19–24. https://doi.org/10.33096/jffi.v9i3.916
  9. Khan, N., & Mukhtar, H. (2018). Tea Polyphenols in Promotion of Human Health. Nutrients, 11(1), 39. https://doi.org/10.3390/nu11010039
  10. Kilmanoglu, H., Yigit Cinar, A., & Durak, M. Z. (2024). Evaluation of microbiota-induced changes in biochemical, sensory properties and volatile profile of kombucha produced by reformed microbial community. Food Chemistry: X, 22, 101469. https://doi.org/10.1016/j.fochx.2024.101469
  11. Kim, H., Hur, S., Lim, J., Jin, K., Yang, T. H., Keehm, I. S., Kim, S. W., Kim, T., & Kim, D. (2023). Enhancement of phenolic compounds and antioxidant activities of kombucha prepared using specific bacterial and yeast. Food Bioscience, 56, 103431. https://doi.org/10.1016/j.fbio.2023.103431
  12. Mendelson, C., Sparkes, S., Merenstein, D. J., Christensen, C., Sharma, V., Desale, S., Auchtung, J. M., Kok, C. R., Hallen-Adams, H. E., & Hutkins, R. (2023). Kombucha tea as an anti-hyperglycemic agent in humans with diabetes: A randomized controlled pilot investigation. Frontiers in Nutrition, 10. https://doi.org/10.3389/fnut.2023.1190248
  13. Mihai, R. A., Cubi-Insuaste, N. S., & Catana, R. D. (2024). Biological activity and phenolic content of kombucha beverages under the influence of different tea extract substrates. Fermentation, 10(7). https://doi.org/10.3390/fermentation10070338
  14. Onsun, B., Toprak, K., & Sanlier, N. (2025). Kombucha tea: A functional beverage and all its aspects. Current Nutrition Reports, 14(1). https://doi.org/10.1007/s13668-025-00658-9
  15. Phan-Van, T., Pham-Quang, H., & Do, A. D. (2024). Cascara kombucha potential as a functional food for fasting glucose regulation, cholesterol control, and liver function modulation: In vitro and in vivo study. International Journal of Food Science and Technology. https://doi.org/10.1111/ijfs.17477
  16. Radeva-Ilieva, M., Stoeva, S., Hvarchanova, N., & Georgiev, K. D. (2025). Green tea: Current knowledge and issues. Foods, 14(5). https://doi.org/10.3390/foods14050745
  17. Santiago-Santiago, R. M., Michel, M. R., Rodríguez-Herrera, R., Aguilar-Zárate, P., Ascacio-Valdés, J. A., & Flores-Gallegos, A. C. (2025). Symbiotic culture of bacteria and yeast (SCOBY) in the food sector as a source of polysaccharides and other applications. Polysaccharides, 6(4), 97. https://doi.org/10.3390/polysaccharides6040097
  18. Shanzet, F. F. F., Karna, N. P. I. S., Khairunnisa, N., Amelia, A., Himawan, A., Darmayasa, I. B. G., & Ramona, Y. (2023). Pengaruh suplementasi buah dan lama fermentasi terhadap mutu organoleptik kombucha teh hitam. Cassowary, 6(1), 9–17. https://doi.org/10.30862/casssowary.cs.v6.i1.194
  19. Silva, M. de F. G. da, Dionísio, A. P., Abreu, F. A. P. de, Brito, E. S. de, Wurlitzer, N. J., Silva, L. M. A. e., Ribeiro, P. R. V., Rodrigues, S., Taniguchi, C. A. K., & Pontes, D. F. (2018). Evaluation of nutritional and chemical composition of yacon syrup using 1H NMR and UPLC-ESI-Q-TOF-MSE. Food Chemistry, 245, 1239–1247. https://doi.org/10.1016/j.foodchem.2017.11.092
  20. Sun, J., Dong, S., Li, J., & Zhao, H. (2022). A comprehensive review on the effects of green tea and its components on immune function. Food Science and Human Wellness, 11(5), 1143–1155. https://doi.org/10.1016/j.fshw.2022.04.008
  21. Tarhan Kuzu, K., Aykut, G., Tek, S., Yatmaz, E., Germec, M., Yavuz, I., & Turhan, I. (2023). Production and characterization of kombucha tea from different sources of tea and its kinetic modeling. Processes, 11(7). https://doi.org/10.3390/pr11072100
  22. Tran, D. B., Le, N. K. N., Duong, M. T., Yuna, K., Pham, L. A. T., Nguyen, Q. C. T., Tragoolpua, Y., Kaewkod, T., & Kamei, K. (2024). Drosophila models of the anti-inflammatory and anti-obesity mechanisms of kombucha tea produced by Camellia sinensis leaf fermentation. Food Science & Nutrition, 12(8), 5722–5733. https://doi.org/10.1002/fsn3.4223
  23. Villarreal-Soto, S. A., Beaufort, S., Bouajila, J., Souchard, J. P., & Taillandier, P. (2018). Understanding kombucha tea fermentation: A review. Journal of Food Science, 83(3), 580–588. https://doi.org/10.1111/1750-3841.14068
  24. Vo, T. P., Ho, T. A. T., Ha, N. M. H., Nguyen, M. T., Chung, M. M., Nguyen, H. N., & Nguyen, D. Q. (2024). Recovering bioactive compounds from yacon (Smallanthus sonchifolius) using ultrasonic-microwave-assisted extraction. Applied Food Research, 4(2). https://doi.org/10.1016/j.afres.2024.100451
  25. Wang, S., Li, C., Wang, Y., Wang, S., Zou, Y., Sun, Z., & Yuan, L. (2023). Changes in physicochemical properties and volatile compounds of Chinese kombucha during fermentation. Food Bioscience, 55, 103029. https://doi.org/10.1016/j.fbio.2023.103029
  26. Xu, S., Wang, Y., Wang, J., & Geng, W. (2022). Kombucha reduces hyperglycemia in type 2 diabetes of mice by regulating gut microbiota and its metabolites. Foods, 11(5). https://doi.org/10.3390/foods11050754

Open Access Copyright (c) 2026 Nabilah Hening Prayitno, Irma Sarita Rahmawati, Fajar Ari Nugroho
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AcTion: Aceh Nutrition Journal
Published by: Department of Nutrition at the Health Polytechnic of Aceh, Ministry of Health.
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