Influence of storage temperature on lipid oxidation kinetics and quality stability of snakehead fish (Channa striata) sausages

Sri Iriyanti* -  Department of Nutrition, Politeknik Kesehatan Jayapura, Jayapura, Indonesia
Budi Kristanto -  Department of Nutrition, Politeknik Kesehatan Jayapura, Jayapura, Indonesia
Nia Budhi Astuti -  1 Department of Nutrition, Politeknik Kesehatan Jayapura, Jayapura, Indonesia
DOI : 10.30867/action.v11i2.3212

Lipid oxidation is the primary cause of quality deterioration in fish-based products, with malondialdehyde (MDA) accumulation posing implications for sensory quality and food safety. This study evaluated the effect of storage temperature on lipid oxidation kinetics in snakehead fish (Channa striata) sausage over 30 d. Room temperature (25 ± 3 °C) and cold storage (10 ± 2 °C) were applied to nine independent samples each. Lipid oxidation was quantified as thiobarbituric acid reactive substances (TBARS) on days 0, 5, 10, 15, 20, 25, and 30, and zero- and first-order kinetic models were evaluated. TBARS increased under both conditions, reaching 0.376 mg MDA/kg at 25 °C and 0.178 mg MDA/kg at 10 °C on day 30, both of which were below the sensory rancidity threshold. Storage temperature, time, and their interaction significantly affected TBARS (p < 0.001; η² = 0.79, 0.85, 0.53). The first-order model fit better at 25 °C (R² = 0.996; k = 0.0598 d ⁻¹), and the zero-order model fit better at 10 °C (R² = 0.981; k = 0.0350 d ⁻¹). The Arrhenius activation energy was measured to be 25.11 kJ/mol. Cold storage reduced the oxidation rate by approximately 41% compared to room temperature, making it a practical option for slowing lipid oxidation in snakehead fish sausage during short-term storage.

Keywords : Lipid oxidation, snakehead fish sausage, TBARS, Arrhenius kinetics, oxidative stability

  1. Bao, Y., & Pignitter, M. (2023). Mechanisms of lipid oxidation in water-in-oil emulsions and oxidomics-guided discovery of targeted protective approaches. Comprehensive Reviews in Food Science and Food Safety, 22(4), 2678–2705.
  2. Brilliantina, A., Wardani, D. K., Fadhila, P. T., Hariono, B., & Wijaya, R. (2022). Accelerated shelf life test method with arrhenius approach for shelf life estimation of tongkol ‘euthynnus affinis’ balado in cans. IOP Conference Series: Earth and Environmental Science, 980(1), 12038. https://doi.org/10.1088/1755-1315/980/1/012038
  3. Farhoosh, R. (2022). New insights into the kinetic and thermodynamic evaluations of lipid peroxidation. Food Chemistry, 375, Article 131659. https://doi.org/10.1016/j.foodchem.2021.131659
  4. Hapsari, R., & Tjandrawinata, R. R. (2025). Nutritional composition and action mechanism of Channa striata meat in wound healing: A systematic review. Narra Journal, 5(3), e2903. https://doi.org/10.52225/narra.v5i3.2903
  6. Husain, R., Suparmo, Harmayani, E., & Hidayat, C. (2016). Kinetic oxidation of protein and fat in snapper (Lutjanus sp) fillet during storage. AIP Conference Proceedings, 1755(1), 50006. https://doi.org/10.1063/1.4958489
  7. Husain, R., Suparmo, S., Harmayani, E., & Hidayat, C. (2018). Komposisi asam lemak, angka peroksida, dan angka TBA fillet ikan kakap (Lutjanus sp.) pada suhu dan lama penyimpanan berbeda. Agritech, 37(3), 319–326. https://doi.org/10.22146/agritech.10529
  8. Iqbal, S., Akram, S., Zafar, S., Shah, S. H. B. U., Saeed, R. F., & Butt, M. S. (2025). Determination of physicochemical properties, oxidative and storage stability of fish and flaxseed oil. Current Research in Nutrition and Food Science Journal, 13(1), 162–171. https://doi.org/10.12944/CRNFSJ.13.1.13
  10. Li, Q., Lei, J., Su, K., Chen, X., Cheng, L., Yang, C., & Ou, S. (2025). Processing and shelf-life prediction models for ready-to-eat crayfish. Foods, 14(8), Article 1296. https://doi.org/10.3390/foods14081296
  11. Maheshwara, K., Naik, J., Balamatti, A., & Jagadish, T. (2017). Biochemical and shelf life study of quality of fish sausage in ambient and refrigerated storage. Biochemical and Cellular Archives, 17(1), 225–230. https://www.cabidigitallibrary.org/doi/full/10.5555/20173175593
  12. Matbo, A., Ghanbari, M. M., Sekhavatizadeh, S. S., & Nikkhah, M. (2025). Stabilizing fish oil during storage with Satureja bachtiarica Bunge. Foods and Raw Materials, 13(2), 254–263. https://doi.org/10.21603/2308-4057-2025-2-254-263
  13. Nguyen, M., Thai, D., & Nguyen, D. (2024). Effects of packaging method and temperature on the quality and lipid degradation of snakehead fish (Channa striata) fillets during frozen storage. Food Research, 8(3), 408–416. https://doi.org/10.26656/fr.2017.8(3).1097
  14. Pasaribu, Y. P., Buyang, Y., Suryaningsih, N. L. S., Dirpan, A., & Djalal, M. (2020). Effect of steaming and pressurized boiling process on the nutrient profile of Papuan cork fish (Channa striata) as a potential protein-rich food to prevent stunting. Medicina Clínica Práctica, 3, 100120. https://doi.org/10.1016/j.mcpsp.2020.100120
  15. Suárez-Medina, M. D., Sáez-Casado, M. I., Martínez-Moya, T., & Rincón-Cervera, M. Á. (2024). The effect of low-temperature storage on the lipid quality of fish, either alone or combined with alternative preservation technologies. Foods, 13(7), Article 1097. https://doi.org/10.3390/foods13071097
  16. Sullivan Ritter, J. C., Budge, S. M., Jovica, F., & Reid, A. J. M. (2015). Oxidation rates of triacylglycerol and ethyl ester fish oils. Journal of the American Oil Chemists' Society, 92(4), 561–569. https://doi.org/10.1007/s11746-015-2626-9
  17. Wang, D., Xiao, H., Lyu, X., Chen, H., & Wei, F. (2023). Lipid oxidation in food science and nutritional health: A comprehensive review. Oil Crop Science, 8(1), 35–44. https://doi.org/10.1016/j.ocsci.2022.11.002
  18. Wang, X., Fan, J., Dong, X., Zhang, S., Yang, Q., Chi, S., ... Tan, B. (2024). Dietary malondialdehyde impairs intestinal health and fillet quality of hybrid grouper (Epinephelus fuscoguttatus ♀ × E. lanceolatus ♂). Animals, 14(22), 3208. https://doi.org/10.3390/ani14223208
  19. Xie, H., Yin, F., Liu, Z., Hu, Y., Yu, M., Zhou, D., & Zhu, B. (2020). Oxidation kinetics of polyunsaturated fatty acids esterified into triacylglycerols and phospholipids in dried scallop (Argopecten irradians) adductor muscles during storage. Food & Function, 11(3), 2349–2357. https://doi.org/10.1039/C9FO02688D
  20. Zakaria, N., & Sarbon, N. (2018). Physicochemical properties and oxidative stability of fish emulsion sausage as influenced by snakehead (Channa striata) protein hydrolysate. LWT, 94, 13–19. https://doi.org/10.1016/j.lwt.2018.04.034

Open Access Copyright (c) 2026 Sri Iriyanti, Budi Kristanto, Nia Budhi Astuti
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AcTion: Aceh Nutrition Journal
Published by: Department of Nutrition at the Health Polytechnic of Aceh, Ministry of Health.
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