Potential of lindur flour (Bruguiera gymnorrhiza) for gluten-free cookies (Kulinur) as an alternative snack for children with autism spectrum disorder

Hidayatusy Syukrina Puteri* -  Nutrition Study Program, Faculty of Health, Mitra Indonesia University, Bandar Lampung, Indonesia
Adhi Nurhartanto -  Nutrition Study Program, Faculty of Health, Mitra Indonesia University, Bandar Lampung, Indonesia
Yunita Dinda Dewi -  Nutrition Study Program, Faculty of Health, Mitra Indonesia University, Bandar Lampung, Indonesia

Autism Spectrum Disorder (ASD) affects about 2.4 million individuals in Indonesia, with 500 new cases annually. Children with ASD often experience gluten and casein intolerance, leading to a restricted diet and inadequate protein and carbohydrate intakes. Lindur flour (Bruguiera gymnorrhiza), containing 2.57–2.68% protein and 80–82% carbohydrates, may serve as a local gluten-free substitute for wheat flour. This quasi-experimental study used a Completely Randomized Design (CRD) with five formulations (F0–F4) combining lindur and modified cassava (mocaf) flours. Proximate composition, gluten content (ELISA), and sensory attributes were analyzed in duplicate and evaluated using one-way ANOVA (p<0.05) and the Kruskal–Wallis test. Increasing the substitution of lindur significantly affected the moisture, ash, protein, fat, crude fiber, and carbohydrate contents. The 100% lindur formulation (F4) exhibited the highest protein (11.54%) and fiber (7.82%) contents, whereas all products were gluten-free (<3 ppm). Sensory evaluation indicated that formulations with 25–50% lindur flour (F1–F2) achieved the highest acceptability in terms of taste, texture, and overall preferences. This study is the first to utilize lindur flour in gluten-free cookie development for children with ASD, demonstrating its potential as a nutritious, locally sourced ingredient for functional food innovation in gluten-free diets

Keywords : Functional Food, Lindur Flour, Gluten-Free, Autism, Cookies

  1. Afifah, D. N., Ningrum, Y. P. A., Syahidah, T., Nuryanto, N., Ayustaningwarno, F., & Sugianto, D. N. (2022). Nutrient content, organoleptic quality, and shelf life of sagon substitute from lindur (Bruguiera gymnorrhiza L.) and soybean flour (Glycine max L.), as an alternative emergency food. Frontiers in Nutrition, 9. https://doi.org/10.3389/fnut.2022.878539
  2. Ahmed, I., Jan, K., Fatma, S., & Dawood, M. A. O. (2022). Muscle proximate composition of various food fish species and their nutritional significance: A review. In Journal of Animal Physiology and Animal Nutrition (Vol. 106, Issue 3, pp. 690–719). John Wiley and Sons Inc. https://doi.org/10.1111/jpn.13711
  3. Amin, M. N. G., Hasan, M. N., Zakariya, Pralebda, S. A., Pramono, H., Saputra, E., Subekti, S., & Alamsjah, M. A. (2019). Texture profile of the bread produced from composite flour Bruguiera gymnorrhiza flour (BGF) and wheat flour. IOP Conference Series: Earth and Environmental Science, 236(1). https://doi.org/10.1088/1755-1315/236/1/012109
  4. Asikin, Y., Nakaza, Y., Oe, M., Kaneda, H., Maeda, G., Takara, K., & Wada, K. (2024). Volatile component composition, retronasal aroma release profile, and sensory characteristics of non-centrifugal cane sugar obtained at different evaporation temperatures. Applied Sciences (Switzerland), 14(24). https://doi.org/10.3390/app142411617
  5. Atmika, C., & Karina, I. A. (2020). Hubungan penerapan diet gluten free casein free dengan perkembangan motorik dan status gizi anak autis di pusat layanan autis kota Denpasar. (Skripsi Sarjana, Poltekkes Denpasar. https://repository.poltekkes-denpasar.ac.id/3989/
  6. Ayu, G., Lestari, D., & Cahyadi, K. D. (2023). Analisis mutu minyak kelapa (VCO) yang diperoleh dari buah kelapa (Cocos nucifera L.). Prosiding Simposium Kesehatan Nasional,2(1). https://doi.org/10.52073/simkesnas.v2i1.71
  7. Cahya P., F., & Berawi, K. N. (2016). Terapi diet bebas gluten dan bebas casein pada Autism Spectrum Disorder (ASD). Majority, 5(1), 38-42. https://adoc.pub/terapi-diet-bebas-gluten-dan-bebas-casein-pada-autism-spectr.html
  8. Chaudhry, T. S., Senapati, S. G., Gadam, S., Mannam, H. P. S. S., Voruganti, H. V., Abbasi, Z., Abhinav, T., Challa, A. B., Pallipamu, N., Bheemisetty, N., & Arunachalam, S. P. (2023). The impact of microbiota on the gut–brain axis: examining the complex interplay and implications. In Journal of Clinical Medicine (Vol. 12, Issue 16). Multidisciplinary Digital Publishing Institute (MDPI). https://doi.org/10.3390/jcm12165231
  9. Dewi, S., & Morawati, S. (2024). Gangguan Autis pada Anak. Scientific Journal(SCIENA), 3(6), 418–431. https://doi.org/https://doi.org/10.56260/sciena.v3i6.177
  10. Gaol, M., Dauly, A., Ridwanto, & Rahayu Yayuk. (2022). Uji kadar protein pada optimasi pembuatan tepung mocaf dari ubi kayu variates roti dan kuning dengan fermentasi lactobacillus casei. Journal Pusat Pendidikan Rakyat, 2(4), 51–60. https://doi.org/10.32696/fjfsk.v2i2.1884
  11. González-Domenech, P. J., Diaz-Atienza, F., Gutiérrez-Rojas, L., Fernández-Soto, M. L., & González-Domenech, C. M. (2022). A narrative review about autism spectrum disorders and exclusion of gluten and casein from the diet. In Nutrients (Vol. 14, Issue 9). MDPI. https://doi.org/10.3390/nu14091797
  12. Hamzah, Y. S., & Fadilla Ratnawaty. (2022). Analisis kandungan tepung buah mangrove jenis lindur (bruigiera sp) sebagai alternatif bahan pangan lokal. Jurnal Pendidikan Tambusai, 6(2). https://doi.org/10.31004/jptam.v7i2.6693
  13. Husain, F., Naiu, A. S., & Dali, F. A. (2023). Pengaruh substitusi tepung buah lindur (Bruguiera gymnorhizza) terhadap karakteristik mutu kue kolombengi. Jurnal Ilmiah Perikanan Dan Kelautan, 11. https://doi.org/10.37905/nj.v11i5.23936
  14. Karim, N., Makbul Syamsuri, A., & Toding, E. (2023). Studi perencanaan bangunan pelindung pantai di Sausapor Kab Tambrauw. Jurnal Teknik Sipil MACCA, 8(1), 37–48. https://doi.org/10.33096/fw6chm58
  15. Kristanti, D., & Setiaboma, W. (2022). Physicochemical and functional properties of composite flours based on mocaf and tempeh flour for making cookies. IIUM Engineering Journal, 23(2), 20–31. https://doi.org/10.31436/iiumej.v23i2.2234
  16. Manik, G. O., Tri, W., & Agustini, R. (2023). Characteristics of baked anchovy brownies (stolephorus spp.) Using api-api mangrove flour (avicennia marina) and mocaf flour. Jurnal Ilmu Dan Teknologi Perikanan, 5(2). https://doi.org/10.14710/jitpi.2023.10679
  17. Masrikhiyah, R. (2021). Retensi kadar gluten cookies substitusi tepung mocaf (Modified Cassava Flour). Jurnal Ilmu Pangan Dan Hasil Pertanian, 5(1), 20–25. https://doi.org/10.26877/jiphp.v5i1.8485
  18. Meidasari, E., Hairudin, Cn, Y., Brajanoto, D., & Bisnis, F. (2023). Pemasaran dodol mangrove melalui digital marketing di desa wisata Pulau Pahawang implementation of pregnancy exercise with gym ball in Padang Pelawi Village, Sukaraja District. Insan Mandiri: Jurnal Pengabdian Masyarakat, 3(1). https://ejournal.stikestrimandirisakti.ac.id/index.php/insanmandiri
  19. Mongdong, P. I. T., Mokosuli, Y. S., & Ogi, N. L. I. M. (2023). The nutritional content found in rhizophora mangrove fruits in Sondaken Village, Tatapaan Subdistrict, South Minahasa. Jurnal Pembelajaran Dan Biologi Nukleus, 9(3), 514–521. https://doi.org/10.36987/jpbn.v9i3.4468
  20. Moreine B. S., Mardiono Jacoeb, A., & Nurhayati, T. (2017). Karakteristik pati dari buah lindur dan aplikasinya sebagai edible film. JPHPI, 20(3), 446–453. https://journal.ipb.ac.id/index.php/jphpi/article/download/19764/13648/0
  21. Nurafifah, D. A., Sumardianto, & Anggo, A. D. (2024). Analisis kadar tanin dengan perbedaan sumber adsorben pada perebusan terhadap tepung mangrove dari buah lindur (Bruguiera Gymnorrhiza). Jurnal Ilmu Dan Teknologi Perikanan, 6(1), 1–8. https://doi.org/10.14710/baf.%25v.%25i.%25Y.144-157
  22. Pebrina, S. M., Wahyuni, S., & Endang Susilowati, P. (2021). Karakteristik fisik biskuit formulasi tepung wikau maombo dengan tepung spirulina plantesis. Jurnal Sains Dan Teknologi Pangan, 6(1). http://dx.doi.org/10.33772/jstp.v6i1.16635
  23. Pentury, M. H. (2016). Analysis of physical and chemical of starch lindur (Bruguiera Gymnorrhiza), pedada (Sonneratia Casiolaris) and api-api (Avicennia Marina). Journal of Environment and Ecology, 7(2), 42. https://doi.org/10.5296/jee.v7i2.10610
  24. Punfujinda, C., Pattarathitiwat, P., Chombhuphan, R., Songnearm, S., Tula, P., Sakkaekaew, K., Anunvrapong, A., Boukaew, S., & Chumkaew, K. (2025). Physical and chemical properties of gluten-free biscuits incorporated with purple sweet potato flour. International Journal of Food Science, 2025(1). https://doi.org/10.1155/ijfo/9955286
  25. Purwoko, A., Julianti, E., Sari, R. K., Widyastuti, D. E., & Sitorus, A. K. (2025). Prospect of processing lindur fruit (Bruguiera sexangula) biscuits as a culinary product to support the development of mangrove-based nature tourism. Food Research, 9(2), 241–251. https://doi.org/10.26656/fr.2017.9(2).110
  26. Rahmaningsih, A., Surti, T., & Anggo Apri Dwi. (2016). Pengaruh Penambahan Tepung Buah Lindur terhadap Kualitas Biskuit Ikan Lele. Jurnal Pengembangan & Biotek, 5(3). https://ejournal3.undip.ac.id/index.php/jpbhp/article/view/16016
  27. Ramadhanty, C., Simanungkalit, S. F., Octaria, Y. C., & Maryusman, T. (2024). Faktor yang berhubuungan dengan status gizi anak dengan Autism Spectrum Disorder di sekolah khusus wilayah JABODETABEK. Amerta Nutrition, 8(2), 206–213. https://doi.org/10.20473/amnt.v8i2.2024.206-213
  28. Raudah, N., & Arif, S. A. (2025). Product development of yellow pumpkin (cucurbita moschata) brownies with the natural sweetener substitution of stevia leaves (stevia rebaudiana) as a functional food. Amerta Nutrition, 9(2), 186–198. https://doi.org/10.20473/amnt.v9i2.2025.186-198
  29. Relita, A., Simamora, M. B., Suroso, E., Rina, O., Hidayati, S., & Silaturahmi. (2024). Sensory and chemical charateristics of cookies based on mangrove type flour (Bruguiera gymnorrizha). Jurnal Agroindustri Berkelanjutan, 3(2). https://doi.org/10.23960/jab.v3i2.9931
  30. Rizky, F. A., Pratiwi, A., Sunarto, S., & Salma Luqyana, N. (2025). Analisis zat gizi dan karakteristik sensoris pukis substitusi tepung mocaf dengan penambahan tepung daun kelor. Jurnal Kesehatan, 13(1), 38–46. https://doi.org/10.25047/j-kes.v13i1.562
  31. Simamora, A. R. M. B., Suroso, E., Rina, O., Hidayati, S., & Silaturahmi, W. (2024). Karakteristik sensori dan kimia cookies berbasis tepung mangrove jenis lindur (bruguiera gymnorrizha) sensory and chemical charateristics of cookies based on mangrove type flour (bruguiera gymnorrizha). Jurnal Agroindustri Berkelanjutan, 3(2), 223–230. https://doi.org/10.23960/jab.v3i2.9931
  32. Sopiandi, R. (2017). Maternal nutrition knowledge, food pattern, dietary intake and nutritional status of children with Autism Spectrum Disorder (ASD). ARGIPA, 2(2), 45–53. https://journal.uhamka.ac.id/index.php/argipa/article/view/2335
  33. Sumartini, S., Harahap, K. S., & Mujiyanti, A. (2021). Brownies from mangrove fruit flour: the use of variation of flours as an alternative to high food nutrition. Indonesian Food and Nutrition Progress, 17(1), 16. https://doi.org/10.22146/ifnp.55188
  34. Viorafanti, O. M., Crosita, Y. O., Malkan Bakhrul Ilmi, I., & Nasrullah, N. (2025). Mocaf flour, moringa flour, and mackerel flour food bars as an emergency food source for neurodivergent/autistic children during a natural disaster. BIO Web of Conferences, 153. https://doi.org/10.1051/bioconf/202515303007
  35. Wibowo, T. D., & Untari, D. S. (2023). Potensi Buah Mangrove dan Ikan Tembakul Sebagai Bahan Alternatif Pembuatan Kaki Naga Ikan. Jurnal Ilmu Perikanan Dan Kelautan, 5(1), 30–45. http://dx.doi.org/10.36526/jl.v5i1.2408
  36. Yu, J. M., Lee, J. H., Park, J. D., Choi, Y. S., Sung, J. M., & Jang, H. W. (2021). Analyzing gluten content in various food products using different types of elisa test kits. Foods, 10(1). https://doi.org/10.3390/foods10010108
  37. Yulia L., R., & Susmayu Saputri, D. (2023). Pengaruh penambahan tepung kulit pisang kepok (musa paradisiacal l) terhadap kadar air, kadar serat kasar dan sifat organoleptik brownies kukus tepung mocaf (Cassava Flour). Food and Agro-Industry Journal, 4(2), 1–8. https://doi.org/10.36761/fagi.v4i2.3550
  38. Zaki, M., Devi, M., & Hidayati, L. (2024). Penggunaan tepung mocaf (modified cassava flour) dengan persentase berbeda mempengaruhi kualitas bolu kukus. Journal of Food Technology and Agroindustry, 6. https://doi.org/10.24929/jfta.v6i1.3363
  39. Zhenilla, M. B., Dewi Masithah, E., & Nirmala, D. (2024). The effect of adding lindur flour (bruguiera gymnorrhiza) on chemical characteristic and acceptability of patin (pangasius sp.) basreng. Journal of Marine and Coastal Science, 13(2), 68–77. https://doi.org/10.20473/jmcs.v13i2.51323

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