Effect of high temperature heating on free fatty acid content and peroxide number of packaged and bulk palm cooking oil
Palm cooking oil is widely used in food processing, particularly for high-temperature frying. Although the thermal degradation of palm oil has been extensively studied, no previous study has specifically compared the degradation rates of bulk and packaged palm oil under extreme heating conditions. Repeated use at high temperatures may induce chemical changes that affect food safety and public health. This study aimed to analyze the effect of heating on changes in free fatty acid (FFA) levels and peroxide value (PV) in palm cooking oil and assess their implications for food quality. An experimental study was conducted in January 2026 at the Chemistry Education Laboratory, University of Bengkulu, using a factorial design with two main factors: oil type (packaged and bulk) and heating temperature (100, 180, and 200°C). Six repetitions were performed at 10-minute intervals over a total heating duration of 60 min. Data were analyzed using the Shapiro–Wilk normality test and two-way ANOVA in SPSS (version 25). At 200°C after 60 min, the packaged oil showed an increase in FFA to 0.31% and PV to 13.6 meqO₂/kg. Bulk oil exhibited more severe deterioration, with FFA and PV reaching 0.37% and 19 meqO₂/kg, respectively. Temperature significantly affected the increase in FFA (p = 0.000) and PV (p = 0.004) values. In conclusion, both parameters exceeded the national safety standards (SNI) threshold. Limiting the repeated use of heated palm oil is essential for protecting consumer health.
Keywords : Palm cooking oil, free fatty acids, peroxide value, high-temperature heating, food safety
- Al Rahmad, A. H. (2021). Faktor risiko obesitas pada guru sekolah perempuan serta relevansi dengan PTM selama pandemi Covid-19. Amerta Nutrition, 5(1), 31–40. https://doi.org/10.20473/amnt.v5i1.2021.31-40
- Anconi, A. C. S. A., Brito, N. C. S., & Nunes, C. A. (2022). Determination of peroxide value in edible oils based on digital image colorimetry. Journal of Food Composition and Analysis, 113, 104724. https://doi.org/10.1016/j.jfca.2022.104724
- Anconi, A. C. S. A., Julia, L. D. J. F., & Cleiton, A. N. (2024). A digital image-based colorimetric method for measuring free acidity in edible vegetable oils. Food Chemistry, 443, 138555. https://doi.org/10.1016/j.foodchem.2024.138555
- Baig, A., Zubair, M., Sumrra, S. H., Nazar, M. F., Zafar, M. N., Jabeen, K., Hassan, M. B., & Rashid, U. (2022). Heating effect on quality characteristics of mixed canola cooking oils. BMC Chemistry, 16(1), 3. https://doi.org/10.1186/s13065-022-00796-z
- Bazina, N., Ahmed, T., Almdaaf, M., Abu Hallalah, H. M. O., & Jibia, S. (2025). Chemical changes in deep-fat frying: Reaction mechanisms, oil degradation, and health implications. Food Science & Nutrition, 13(10), e70969. https://doi.org/10.1002/fsn3.70969
- Dangal, A., Tahergorabi, R., Acharya, D. R., Timsina, P., Rai, K., Dahal, S., Acharya, P., & Giuffrè, A. M. (2024). Review on deep-fat fried foods: Physical and chemical attributes, and consequences of high consumption. European Food Research and Technology, 250(6), 1537–1550. https://doi.org/10.1007/s00217-024-04482-3
- Di Pietro, M. E., Mannu, A., & Mele, A. (2020). NMR determination of free fatty acids in vegetable oils. Processes, 8(4), 410. https://doi.org/10.3390/pr8040410
- Domínguez, R., Pateiro, M., Gagaoua, M., Barba, F. J., Zhang, W., & Lorenzo, J. M. (2019). A comprehensive review on lipid oxidation in meat and meat products. Antioxidants, 8(10), 429. https://doi.org/10.3390/antiox8100429
- Dwiecki, K., Błaszczak, E., Dubois, E., Lecerf, J., & Viau, P. (2026). Recent advances in lipid oxidation of bulk oils: Current insights and unresolved challenges. Journal of Future Foods. https://doi.org/10.1016/j.cocis.2026.102000
- Falade, A. O., Oboh, G., & Okoh, A. I. (2017). Potential health implications of the consumption of thermally-oxidized cooking oils: A review. Polish Journal of Food and Nutrition Sciences, 67(2). https://doi.org/10.1515/pjfns-2016-0028
- Flores, M., Avendaño, V., Bravo, J., Valdés, C., Forero-Doria, O., Quitral, V., Vilcanqui, Y., & Ortiz-Viedma, J. (2021). Edible oil parameters during deterioration processes. International Journal of Food Science, 2021(1), 7105170. https://doi.org/10.1155/2021/7105170
- Ganesan, K., Sukalingam, K., & Xu, B. (2019). Impact of consumption of repeatedly heated cooking oils on the incidence of various cancers: A critical review. Critical Reviews in Food Science and Nutrition, 59(3), 488–505. https://doi.org/10.1080/10408398.2017.1379470
- Gharby, S., Asbbane, A., Nid Ahmed, M., Gagour, J., Hallouch, O., Oubannin, S., Bijla, L., Goh, K. W., Bouyahya, A., & Ibourki, M. (2025). Vegetable oil oxidation: Mechanisms, impacts on quality, and approaches to enhance shelf life. Food Chemistry: X, 28, 102541. https://doi.org/10.1016/j.fochx.2025.102541
- González-Torres, P., García-Ruiz, Á., & La Rubia, M. D. (2025). Impact of thermal treatment and storage on degradation and migration in edible seed oils packaged in PET. Journal of Stored Products Research, 113, 102679. https://doi.org/10.1016/j.jspr.2025.102679
- Hidayatno, A., Setiawan, A. D., Subroto, A., Saheruddin, H., Wardono, S., Romijn, H., Zahari, T. N., Rahman, I., Jafino, B. A., Moeis, A. O., Komarudin, K., Fitriani, A. R., Julio, N., & Zafira, Z. (2025). Exploring the food-versus-fuel debate in Indonesia’s palm oil industry toward sustainability: A model-based policymaking approach. Energy Nexus, 19, 100511. https://doi.org/10.1016/j.nexus.2025.100511
- Hidayati, J. P., Hariyadi, A., & Chosta, F. (2022). Unjuk kinerja adsorpsi bentonit dan arang aktif terhadap karakteristik minyak jelantah. Jurnal Sains dan Teknologi Pangan, 7(6), 5600–5614.
- Hidayati, S., Rahmawati, A., Suroso, E., Subeki, S., & Utomo, T. P. (2025). Impact of high-temperature heating on the chemical stability and sensory quality of red palm oil. Jurnal Teknik Pertanian Lampung (Journal of Agricultural Engineering), 14(1), 215–225. https://doi.org/10.23960/jtep-l.v14i1.215-225
- Husain, F., & Marzuki, I. (2021). Pengaruh temperatur penyimpanan terhadap mutu dan kualitas minyak goreng kelapa sawit. Jurnal Serambi Engineering, 6(4), 2270–2278. https://doi.org/10.32672/jse.v6i4.3470
- Kmiecik, D., Fedko, M., Siger, A., & Kulczyński, B. (2019). Degradation of tocopherol molecules and its impact on the polymerization of triacylglycerols during heat treatment of oil. Molecules, 24(24), 4555. https://doi.org/10.3390/molecules24244555
- Kusumawaty, Y., Edwina, S., & Sifqiani, N. S. (2019). Sikap dan perilaku konsumen minyak goreng curah dan kemasan di Kota Pekanbaru. Jurnal Ecodemica, (2), 111–122. http://ejournal.bsi.ac.id/ejurnal/index.php/ecodemica
- Liang, S., Chen, G., Ma, C., Zhu, C., Li, L., Gao, H., & Yang, T. (2025). Quantitative determination of acid value in palm oil during thermal oxidation using Raman spectroscopy combined with deep learning models. Food Chemistry, 474, 143107. https://doi.org/10.1016/j.foodchem.2025.143107
- Lika, L. C. R., Luhtansa, S. S., Blaon, S. B., & Panjaitan, R. S. (2022). Comparison of acid numbers in bulk and packaged cooking oil samples. Indonesian Journal of Pharmaceutical Research, 2(2), 22–26. https://doi.org/10.31869/ijpr.v2i2.4155
- MacArthur, R., Teye, E., & Darkwa, S. (2021). Quality and safety evaluation of important parameters in palm oil from major cities in Ghana. Scientific African, 13, e00860. https://doi.org/10.1016/j.sciaf.2021.e00860
- Manurung, M. M., Suaniti, N. M., & Dharma Putra, K. G. (2018). Perubahan kualitas minyak goreng akibat lamanya pemanasan. Jurnal Kimia, 59. https://doi.org/10.24843/jchem.2018.v12.i01.p11
- Moghadas, H. C., Chauhan, R., & Smith, J. S. (2024). Application of plant oils as functional additives in edible films and coatings for food packaging: A review. Foods, 13(7), 997. https://doi.org/10.3390/foods13070997
- Nico, N. A. P., & Azara, R. (2021). Comparative of the quality of cooking oil with four times frying on packaged and bulk cooking oil: Perbandingan kualitas minyak goreng dengan empat kali penggorengan pada minyak goreng kemasan dan curah. Journal of Tropical Food and Agroindustrial Technology, 2(1), 9–14. https://doi.org/10.21070/jtfat.v2i01.1576
- Nurrahmah, A., & Putri, S. R. F. Y. (2020). Analisis perbandingan penggunaan minyak curah dan minyak kemasan menggunakan uji hipotesis dua proporsi. Bulletin of Applied Industrial Engineering Theory, 1(2). https://jim.unindra.ac.id/index.php/baiet/article/view/2846
- Pramitha, D. A. I., et al. (2022). Kualitas minyak oles yang diproduksi dari virgin coconut oil (VCO) dan bunga cengkeh dengan variasi suhu pemanasan. Jurnal Kimia, 16(2). https://doi.org/10.24843/JCHEM.2022.v16.i02.p04
- Putra, E. Y. G., Inul, L. F., Adnan, K. N., & Andi, N. (2024). Pengaruh waktu penyimpanan dan kontaminasi bleaching earth, spent bleaching earth dan bleaching earth teraktivasi terhadap bilangan peroksida refined bleached deodorized palm oil. Seminar Nasional Pengkajian dan Penerapan Sains Teknologi, 1(1), 1–7.
- Qothrunnada, L., Yanti, I., Pauzan, M., Wiralodra, U., & Fuzzy, L. (2024). Implementasi logika fuzzy pada alat pendeteksi kualitas minyak goreng berdasarkan pH dan tingkat kejernihan: Implementation of fuzzy logic in cooking oil quality detection device based on pH and clarity level. Jurnal Teknologi Informasi dan Ilmu Komputer (JTIIK), 11(1). https://doi.org/10.25126/jtiik.20241118289
- Rahman, Y., & Farpina, E. (2024). Analysis of the effect of palm cooking oil storage after frying on the number of peroxides with iodometric titration method. MMLTJ (Mahakam Medical Laboratory Technology Journal), 4(1), 54–63. https://ejournalanalis.poltekkes-kaltim.ac.id/ojs/index.php/Analis/article/view/209
- Sari, A. M., Pandit, A. W., & Abdullah, S. (2021). Pengaruh variasi massa karbon aktif dari limbah kulit durian (Durio zibethinus) sebagai adsorben dalam menurunkan bilangan peroksida dan bilangan asam pada minyak goreng bekas. Jurnal Konversi, 10(1), 1–7. https://jurnal.umj.ac.id/index.php/konversi/article/view/10238
- Setyobudi, R. H., Anwar, S., Garfansa, M. P., Liwang, T., Iswahyudi, I., Damat, D., … Dewi, I. O. (2024). Microplastic debris in palm cooking oil: A call for research. In BIO Web of Conferences (Vol. 104, Article 00037). EDP Sciences. https://doi.org/10.1051/bioconf/202410400037
- Syafrizal, Pevi, R., & Selfa, D. S. (2023). Studi perbedaan kualitas produksi minyak goreng kemasan A dan B serta curah. Media, 15(2), 66–77. https://doi.org/10.33506/md.v15i2.2383
- Syaputra, R., & Sofiyanurriyanti, S. S. (2022). Analisis pengendalian mutu pada asam lemak bebas minyak kelapa sawit menggunakan metode SQC. Jurnal Teknik Industri: Jurnal Hasil Penelitian dan Karya Ilmiah dalam Bidang Teknik Industri, 8(1), 59–66. https://doi.org/10.24014/jti.v8i1.15488
- Tan, B. A., Nair, A., Zakaria, M. I. S., Low, J. Y. S., Kua, S. F., Koo, K. L., Wong, Y. C., Neoh, B. K., Lim, C. M., & Appleton, D. R. (2023). Free fatty acid formation points in palm oil processing and the impact on oil quality. Agriculture, 13(5), 957. https://doi.org/10.3390/agriculture13050957
- Wang, T.-W., Liu, J.-H., Tsou, H.-H., Liu, T.-Y., & Wang, H.-T. (2019). Identification of acrolein metabolites in human buccal cells, blood, and urine after consumption of commercial fried food. Food Science & Nutrition, 7(5), 1668–1676. https://doi.org/10.1002/fsn3.1001
- Zhang, Q., Chen, B., Ma, P., Yi, L., Gu, Y., & Wang, S. (2026). Emerging insights into lipid oxidation in dry-cured meats: Adverse effect on product quality and safety. Journal of Future Foods, 658 (January). https://doi.org/10.1016/j.jfutfo.2026.01.034
- Zuliyama, J., Rahmanpiu, & Wa, O. M. (2023). Deskripsi kualitas minyak goreng hasil pemanasan. Sains: Journal of Chemistry and Chemistry Education, 12(1), 57–63. https://doi.org/10.36709/sains.v12i1.34
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