Efektivitas pertumbuhan Candida albicans pada media alternatif air rebusan kacang kedelai (Glycine max (l) Merr)

Author(s): Rahmayanti Rahmayanti, Siti Hadijah, Syarifah Wahyuni, Safwan Safwan
DOI: 10.30867/gikes.v4i1.1067


Background: Candida albicans are one of the pathogenic fungi in humans whose growth requires carbohydrates and proteins to grow and develop. Soybeans have a reasonably good nutritional content and potential as an alternative medium for the growth of Candida albicans fungi, and soybeans are very easy to find in the community, so they can be used as an alternative medium to replace SGA (Sabouraud Glucose Agar). Soybeans (Glycine max (L.) Merr) have nutritional content consisting of oils, carbohydrates, and minerals that allow them to source nutrients and food for fungi. Furthermore, one of them that can be utilized from soybeans is cooking water.

Objectives: The study aims to determine whether soybean (Glycine max (L.) Merr) cooking water media can be an effective alternative for Candida albicans culture.

Methods: An analytical study using an experimental design was conducted in the Microbiology laboratory at the Department of Medical Laboratory Technology, Health Polytechnic of Aceh, Ministry of Health, in September 2022. The sample used in this study was soybean cooking water as an alternative medium for Candida albicans. Data collection is based on the culture (breeding) of Candida albicans on alternative media with soybean decoction water as raw material. For the identification of Candida albicans species, confirmation tests were carried out using the germ tube test method. Then the growth assessment of positive (+) colonies of Candida albicans on alternative media of soybean cooking water was incubated for 72 hours (± three days) at 37°C. Data analysis was only done descriptively

Results: There is the growth of Candida albicans fungal colonies on alternative media from soybean cooking water with macroscopic characteristics of yellowish white color, yeast smell, smooth, smooth surface, flat edges, small colonies, and many colonies. On microscopic observation, Candida albicans fungus was found with microscopic characteristics of round, oval, small, thin-walled, yeast-like cells and pseudohyphae.

Conclusion: Soybean (Glycine max (L) Merr) cooking water media is effectively used as an alternative media for the growth of Candida albicans.


Boiled water; Candida albicans; media; soybean

Full Text:



Anisah. (2015). Media alternatif untuk pertumbuhan bakteri menggunakan sumber karbohidrat yang berbeda. In Universitas Muhammadiyah Surakarta. Universitas Muhammadiyah Surakarta.

Chang, C.-K., Yang, M.-C., Chen, H.-F., Liao, Y.-L., & Lan, C.-Y. (2022). The Role of Sfp1 in Candida albicans Cell Wall Maintenance. In Journal of Fungi (Vol. 8, Issue 11). https://doi.org/10.3390/jof8111196

Echeverria-Jaramillo, E., Kim, Y., Nam, Y., Zheng, Y., Cho, J. Y., Hong, W. S., Kang, S. J., Kim, J. H., Shim, Y. Y., & Shin, W.-S. (2021). Revalorization of the cooking water (Aquafaba) from soybean varieties generated as a by-product of food manufacturing in Korea. Foods, 10(10), 2287. https://doi.org/10.3390/foods10102287

Hajjeh, R. A., Sofair, A. N., Harrison, L. H., Lyon, G. M., Arthington-Skaggs, B. A., Mirza, S. A., Phelan, M., Morgan, J., Lee-Yang, W., & Ciblak, M. A. (2004). Incidence of bloodstream infections due to Candida species and in vitro susceptibilities of isolates collected from 1998 to 2000 in a population-based active surveillance program. Journal of Clinical Microbiology, 42(4), 1519–1527. https://doi.org/10.1128/JCM.42.4.1519-1527.2004

Irianto, K. (2014). Bakteriologi medis, mikologi medis, dan virologi medis. In Alfabeta. Alfabeta.

Jawetz, E., Melnick, J., Adelberg, E., Brooks, G., Butel, J., & Ornston, L. (2013). Mikrobiologi Kedokteran. In Nugroho & R. F. Maulany (Eds.), Penerbit Buku Kedokteran EGC (Edisi ke-2). Penerbit Buku Kedokteran EGC.

Lin, Y., & Wu, S. (2021). Vegetable soybean (Glycine max (L.) Merr.) leaf extracts: Functional components and antioxidant and anti‐inflammatory activities. Journal of Food Science, 86(6), 2468–2480.

Logo, N. J. B., Zubaidah, S., & Kuswantoro, H. (2018). Karakteristik Morfologi Polong Beberapa Genotipe Kedelai (Glycine max L. Merill). Seminar Hayati V Tahun 2017.

Mayer, F. L., Wilson, D., & Hube, B. (2013). Candida albicans pathogenicity mechanisms. Virulence, 4(2), 119–128. https://doi.org/10.4161/viru.22913

Moran, G. P., Coleman, D. C., & Sullivan, D. J. (2012). Candida albicans versus Candida dubliniensis: Why Is C. albicans More Pathogenic? International Journal of Microbiology, 2012, 205921. https://doi.org/10.1155/2012/205921

Ningrum, N. R., Widhorini, W., & Yuliani, E. (2018). Analisis Pertumbuhan Jamur Aspergillus fumigatus dalam Media Kacang Hijau (Phaseolus radiatus L.). Jurnal Kesehatan Kartika, 8(1), 15–25.

Nuryati, A. (2017). Efektivitas Berbagai Konsentrasi Kacang Kedelai (Glycine max (L.) Merill) Sebagai Media Alternatif Terhadap Pertumbuhan Jamur Candida albicans. Jurnal Teknologi Laboratorium, 5(1), 1–4.

Ponde, N. O., Lortal, L., Ramage, G., Naglik, J. R., & Richardson, J. P. (2021). Candida albicans biofilms and polymicrobial interactions. Critical Reviews in Microbiology, 47(1), 91–111. https://doi.org/10.1080/1040841X.2020.1843400

Prayitno, T. A. (2017). Pengembangan petunjuk praktikum mikrobiologi program studi pendidikan biologi. Jurnal Biota, 3(1), 31–37.

Putra, S. F., Fitri, R., & Fadilah, M. (2022). Pembuatan Media Tumbuh Bakteri Berbasis Lokal Material. Prosiding Seminar Nasional Biologi, 1(2), 1043–1050.

Ravimannan, N., Arulanantham, R., Pathmanathan, S., & Niranjan, K. (2014). Alternative culture media for fungal growth using different formulation of protein sources. Annals of Biological Research, 5(1), 36–39.

Roosheroe, G. L., Sjamsuridzal, W., & Oetati, A. (2014). Mikologi dasar dan terapan. In Yayasan Pustaka Obor Indonesia. Yayasan Pustaka Obor Indonesia.

Ruhnke, M. (2006). Epidemiology of Candida albicans infections and role of non-Candidaalbicans yeasts. Current Drug Targets, 7(4), 495–504.

Sakimoto, K. K., Kornienko, N., Cestellos-Blanco, S., Lim, J., Liu, C., & Yang, P. (2018). Physical Biology of the Materials–Microorganism Interface. Journal of the American Chemical Society, 140(6), 1978–1985. https://doi.org/10.1021/jacs.7b11135

Schoeters, F., & Van Dijck, P. (2019). Protein-protein interactions in Candida albicans. Frontiers in Microbiology, 1792. https://doi.org/10.3389/fmicb.2019.01792

Sjam, K. R. (2012). Kolonisasi Candida dalam rongga mulut. Majalah Kedokteran UKI, 28(1), 39–47.

Talapko, J., Juzbašić, M., Matijević, T., Pustijanac, E., Bekić, S., Kotris, I., & Škrlec, I. (2021). Candida albicans—the virulence factors and clinical manifestations of infection. Journal of Fungi, 7(2), 79. https://doi.org/10.3390/jof7020079


  • There are currently no refbacks.