Potensi antimikroba minyak atsiri daun jeruk (Citrus)

Author(s): Munira Munira
DOI: 10.30867/gikes.v1i1.290

Abstract

Background: Some plants that are included in the genus Citrus are one of the plants producing essential oils which is a natural substance that is known to have an antibacterial effect.

Objectives: The study aims to determine the ability of essential oils from the leaves of several types of citrus (Citrus) in inhibiting the growth of Escherichia coli.

Methods: An experimental study using a complete randomized design consisting of 4 treatments and four replications each. The treatments consisted of distilled water (control), lime leaf essential oil, kaffir lime essential oil and lime leaf essential oil. Microbiology test uses agar diffusion method.

Results: Anova test showed that essential oils from some lime can inhibit the growth of Escherichia coli (p = 0.000). Duncan's further test results showed that the average diameter of the inhibition zone formed showed that the average diameter of the largest inhibition zone was formed in the treatment which was given lime orange essential oil (16.55 mm) and was not significantly different (p <0.05) with orange essential oil kaffir (15.40 mm) and lime essential oil (14.97 mm). Based on the Duncan test results showed that there is no difference in the average diameter of inhibitory zones between essential oils of lime, lime and lime, this can be caused because the three lime are classified in the same genus, namely the genus citrus.

Conclusion: Essential oils from some lime can inhibit the growth of Escherichia coli. The average diameter of the inhibition zone that formed showed the greatest inhibition formed in the treatment which was given lime essential oil.

Keywords

Citrus; essential oils; Escherichia coli

Full Text:

PDF

References

Ajizah, A. (2018). Sensitivitas Salmonella tyhimurium terhadap ekstrak daun Psidium guajava L. Bioscientiae, 1(1), 31–38.

Armando, R. (2009). Memproduksi 15 Minyak Atsiri Berkualitas. Penebar Swadaya Grup.

Chutia, M., Bhuyan, P. D., Pathak, M. G., Sarma, T. C., & Boruah, P. (2009). Antifungal activity and chemical composition of Citrus reticulata Blanco essential oil against phytopathogens from North East India. LWT-Food Science and Technology, 42(3), 777–780.

Fadlilah, M. (2015). Benefit of Red Betel (Piper crocatum Ruiz & Pav.) as Antibiotics. Jurnal Majority, 4(3), 71–75.

Grafton-Cardwell, E. E., Stelinski, L. L., & Stansly, P. A. (2013). Biology and management of Asian citrus psyllid, vector of the huanglongbing pathogens. Annual Review of Entomology, 58, 413–432.

Hertiani, T., Palupi, I. S., Sanliferianti, & Nurwindasari, H. D. (2003). Uji Potensi Antimikroba terhadap S. aureus, E. coli, Shigella dysentriae dan Candida albicans dari Beberapa Tanaman Obat Tradisional untuk Penyakit Infeksi. Pharmacon, 4(2).

Jawetz, E., Melnick, J., Adelberg, E., Brooks, G., Butel, J., & Ornston, L. (2005). Mikrobiologi Kedokteran (Nugroho & R. F. Maulany (eds.); Edisi ke-2). Penerbit Buku Kedokteran EGC.

Jenssen, H., Hamill, P., & Hancock, R. E. W. (2006). Peptide antimicrobial agents. Clinical Microbiology Reviews, 19(3), 491–511.

Kan, Y., Uçan, U. S., Kartal, M., Altun, M. L., Aslan, S., Sayar, E., & Ceyhan, T. (2006). GC-MS analysis and antibacterial activity of cultivated Satureja cuneifolia Ten. essential oil. Turkish Journal of Chemistry, 30(2), 253–259.

Kharismayanti, A. (2015). Uji Aktivitas Antibakteri Minyak Atsiri Daun Jeruk Nipis (Citrus aurantifolia (Christm. & Panz.) Swingle) Terhadap Porphyromonas gingivalis ATCC 33277 Secara In Vitro [University of Jember]. In Faculty of Dentistry,. https://repository.unej.ac.id/handle/123456789/65611

Loh, F. S., Awang, R. M., Omar, D., & Rahmani, M. (2011). Insecticidal properties of Citrus hystrix DC leaves essential oil against Spodoptera litura fabricius. Journal of Medicinal Plants Research, 5(16), 3739–3744.

Marsono, Y. (2008). Prospek pengembangan makanan fungsional. Jurnal Teknologi Pangan Dan Gizi, 7(1), 19–27.

Morales, G., Sierra, P., Mancilla, A., PAREDES, A., LOYOLA, L. A., GALLARDO, O., & BORQUEZ, J. (2003). Secondary metabolites from four medicinal plants from northern Chile: antimicrobial activity and biotoxicity against Artemia salina. Journal of the Chilean Chemical Society, 48(2), 13–18.

Pandey, A. K., & Kumar, S. (2013). Perspective on plant products as antimicrobial agents: A review. Pharmacologia, 4(7), 469–480.

Rosyad, P. G. Y. (2009). Formulasi Gel Obat Jerawat Minyak Atsiri Daun Jeruk Nipis (Citrus aurantifolia, Swingle) dan Uji Daya Antibakteri (Propionibacterium acne) Secara In Vitro [Universitas Muhammadiyah Surakarta]. In Universitas Muhammadiyah Surakarta. http://eprints.ums.ac.id/3378/

Salni, S., Marisa, H., & Mukti, R. W. (2011). Isolasi senyawa antibakteri dari daun jengkol (Pithecolobium lobatum benth) dan penentuan nilai KHM-nya. Jurnal Penelitian Sains, 14(1), 38–41. https://doi.org/https://doi.org/10.36706/jps.v14i1.125

Widharto, W. (2019). Tanaman dalam Manuskrip Indonesia sebagai Bahan Rujukan Penemuan Obat Baru. Jumantara: Jurnal Manuskrip Nusantara, 2(2), 154–180.

Yuliani, R., Indrayudha, P., & Rahmi, S. S. (2011). Aktivitas antibakteri minyak atsiri daun jeruk purut (Citrus hystrix) terhadap Staphylococcus aureus dan Escherichia coli. Pharmacon, 12(2), 101–110.

DOI: http://dx.doi.org/10.30867/gikes.v1i1.290

Refbacks

  • There are currently no refbacks.