Formulasi minuman sinbiotik dengan okara (Glycine max) dan probiotik Lactiplantibacillus plantarum Dad-13

Author(s): Angelina Christine Suplig, Ida Bagus Agung Yogeswara, Purwaningtyas Kusumaningsih, Dylla Hanggaeni Dyah Puspaningrum
DOI: 10.30867/gikes.v5i3B.1818

Abstract

Background: Okara is the byproduct of soymilk production process. Okara contains high nutritional value and considered as a source of oligosaccharide. Oligosaccharide in okara will stimulate the growth of probiotic bacteria and thus showed prebiotic effect. The underutilization of goat milk can be used as a carrier for probiotic bacteria as well as to develop sinbiotic functional foods.

Objective: This study aims to determine the formulation of fermented goat's milk with the addition of okara (glycine max) as a synbiotic drink to obtain dietary fibre, amino acid profiles as well as microbiology and sensory profiles.

Methods: Randomized complete designed were used in this study with 5 formulations with the addition of okara. The study was conducted in Laboratorium Sains Dasar Universitas Dhyana Pura in July of 2023. The okara was obtained at local tofu producer in Denpasar, whereas goat milk was obtained from milk distributor located in Denpasar. The populations of this study were okara and goat milk that already thoroughly cleaned and pasteurised. Data collection was performed in the laboratory and all data were analyzed using Anova and Duncan with significant levels of 95%.

Results: The study showed that the addition of 5% okara (F4) (Glycine max) was the most prefarable symbiotic drink. Based on proximate analysis, the F4 contained water 88.58%, fat 1.11%, protein 2.17%, carbohydrate 7.656%, total LAB 5.05 x 108 CFU/ mL, essential amino acids, and 2.19% dietary fiber. Based on sensory tests, formulation 1 (0%) and formulation 4 (5%) had overall significant differences.

Conclusion: The addition of okara 5% with goat's milk can be used as a synbiotic drink. The formulation with the addition of 0% okara and 5% okara have overall significant differences in terms of taste, texture, aroma and also color.

Keywords

Fermentation, okara, synbiotics, goat milk, probiotic

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References

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Islam, M. Z., Uddin, M. E., Rahman, M. T., Islam, M. A., & Harun-ur-Rashid, M. (2021). Isolation and characterization of dominant lactic acid bacteria from raw goat milk: Assessment of probiotic potential and technological properties. Small Ruminant Research, 205, 106532. https://doi.org/https://doi.org/10.1016/j.smallrumres.2021.106532

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Nurkhoeriyati, T., & Iswaldi, I. (2019). Peningkatan isoflavon dalam produk berbasis okara yang difermentasi oleh jamur. Jurnal Dunia Gizi, 2(1), 28–35. https://doi.org/10.33085/jdg.v2i1.4146

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Rochmah, S., Yani, A., & Aminarista. (2018). Kadar protein dan daya terima silky pudding yang disubstitusi susu kambing dan susu skim sebagai alternatif makanan tambahan balita stunting. Journal of Holistic and Health Sciences, 2(2), 72–77. https://doi.org/10.51873/jhhs.v2i2.29

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Susilawati, Zulferiyenni, Sari, P., & Sartika, D. (2017). Penyuluhan dan pelatihan pembuatan kefir susu kambing dalam rangka meningkatkan gizi masyarakat di Desa Sumber Rejo Kecamatan Jati Agung Kabupaten Lampung Selatan. Jurnal Sakai Sambayan, 1(3), 140–144.

Utami, R., Widowati, E., & Dewati, A. D. A. R. (2013). Kajian penggunaan tepung gembili (Dioscorea esculenta) dalam pembuatan minuman sinbiotik terhadap total bakteri probiotik, karakter mutu, dan karakter sensoris. Jurnal Teknosains Pangan, 2(3), 3–8.

Vong, W. C., & Liu, S. Q. (2016). Biovalorisation of okara (soybean residue) for food and nutrition. Trends in Food Science and Technology, 52, 139–147. https://doi.org/10.1016/j.tifs.2016.04.011

Yang, Q., Zhao, D., Zhang, C., Sreenivasulu, N., Sun, S. S.-M., & Liu, Q. (2022). Lysine biofortification of crops to promote sustained human health in the 21st century. Journal of Experimental Botany, 73(5), 1258–1267. https://doi.org/10.1093/jxb/erab482

Yoshida, B. Y., & Prudencio, S. H. (2020). PHysical, chemical, and technofunctional properties of okara modified by a carbohydrase mixture. LWT, 13(4), 110–141. https://doi.org/https://doi.org/10.1016/j.lwt.2020.110141

Aflatoonian, M., Taghavi Ardakani, A., Modarresi, S. Z., Modaresi, V., Karimi, M., Ordooei, M., Vakili, M., & Pakseresht, B. (2020). The Effect of Synbiotic Supplementation on Growth Parameters in Mild to Moderate FTT Children Aged 2–5 Years. Probiotics and Antimicrobial Proteins, 12(1), 119–124. https://doi.org/10.1007/s12602-018-9508-6

Ahmed, I., Qaisrani, S. N., Azam, F., Pasha, T. N., Bibi, F., Naveed, S., & Murtaza, S. (2020). Interactive effects of threonine levels and protein source on growth performance and carcass traits, gut morpHology, ileal digestibility of protein and amino acids, and immunity in broilers. Poultry Science, 99(1), 280–289. https://doi.org/https://doi.org/10.3382/ps/pez488

Bahrami, M., Morris, M. B., & Day, M. L. (2023). Glutamine, proline, and isoleucine support maturation and fertilisation of bovine oocytes. Theriogenology, 201, 59–67. https://doi.org/https://doi.org/10.1016/j.theriogenology.2023.02.019

Beal, T., Tumilowicz, A., Sutrisna, A., Izwardy, D., & Neufeld, L. M. (2018). A review of child stunting determinants in Indonesia. Maternal and Child Nutrition, 14(4), 1–10. https://doi.org/10.1111/mcn.12617

Bishop, C. A., Schulze, M. B., Klaus, S., & Weitkunat, K. (2020). The branched-chain amino acids valine and leucine have differential effects on hepatic lipid metabolism. The FASEB Journal, 34(7), 9727–9739. https://doi.org/https://doi.org/10.1096/fj.202000195R

Chen, X., Zheng, R., Liu, R., & Li, L. (2020). Goat milk fermented by lactic acid bacteria modulates small intestinal microbiota and immune responses. Journal of Functional Foods, 65, 103744. https://doi.org/https://doi.org/10.1016/j.jff.2019.103744

da Silva, L. A., Lopes Neto, J. H. P., & Cardarelli, H. R. (2019). Safety and probiotic functionality of isolated goat milk lactic acid bacteria. Annals of Microbiology, 69(13), 1497–1505. https://doi.org/10.1007/s13213-019-01533-z

Devita, M., Rizqiati, H., & Pramono, Y. (2019a). Pengaruh Lama Fermentasi Terhadap Kadar Alkohol, Lemak, Nilai pH, dan Total BAL Kefir Prima Susu Kambing. Jurnal Teknologi Pangan, 3(2), 204–208. https://doi.org/https://doi.org/10.14710/jtp.2019.23772

Devita, M., Rizqiati, H., & Pramono, Y. B. (2019b). Pengaruh lama fermentasi terhadap kadar alkohol, lemak, nilai pH, dan total BAL kefir prima susu kambing. Jurnal Teknologi Pangan, 3(2), 204–208.

Dimitrellou, D., Salamoura, C., Kontogianni, A., Katsipi, D., Kandylis, P., Zakynthinos, G., & Varzakas, T. (2019). Effect of Milk Type on the Microbiological, PHysicochemical and Sensory Characteristics of Probiotic Fermented Milk. In Microorganisms (Vol. 7, Issue 9, p. 1322). https://doi.org/10.3390/microorganisms7090274

Flynn, N. E., Shaw, M. H., & Becker, J. T. (2020). Amino Acids in Health and Endocrine Function BT - Amino Acids in Nutrition and Health: Amino acids in systems function and health (G. Wu (ed.); pp. 97–109). Springer International Publishing. https://doi.org/10.1007/978-3-030-45328-2_6

Fuady, M. S., & Sumantri, J. (2021). Model bisnis peternakan kambing perah rakyat di lingkungan penyandang disabilitas. Jurnalku, 1(4), 312–322. https://doi.org/10.54957/jurnalku.v1i4.75

Gunawan, G., Fadlyana, E., & Rusmil, K. (2011). Hubungan status gizi dan perkembangan anak usia 1 - 2 tahun. Sari Pediatri, 13(2), 142–146. https://doi.org/10.14238/sp13.2.2011.142-6

Harjanti, D. W., & Kusumaningrum, D. G. (2021). Pengaruh Lama Pemaparan Ozon Terhadap Kualitas Mikrobiologi dan Kandungan Nutrisi Susu Kambing Peranakan Ettawa. Jurnal Aplikasi Teknologi Pangan, 10(1), 1–5. https://doi.org/https://doi.org/10.17728/jatp.7252

Haryadi, Nurliana, & Sugito. (2013). Nilai pH dan jumlah bakteri asam laktat kefir susu kambing setelah difermentasi dengan penambahan gula dengan lama inkubasi yang berbeda. JurnalMedika Veterinaria, 7(1), 4–7. https://doi.org/https://doi.org/10.21157/j.med.vet..v7i1.2905

Huang, Z., Huang, L., Xing, G., Xu, X., Tu, C., & Dong, M. (2020). Effect of Co-Fermentation with Lactic Acid Bacteria and K. marxianus on PHysicochemical and Sensory Properties of Goat Milk. In Foods (Vol. 9, Issue 3, p. 1921). https://doi.org/10.3390/foods9030299

Ibrahim, Rasha, Essawy, ;, El-Kenany, E. ;, & El-Nawawy. (2016). Preparation of synbiotic sport drinks using sweet whey and milk permeate. January 2016.

Islam, M. Z., Uddin, M. E., Rahman, M. T., Islam, M. A., & Harun-ur-Rashid, M. (2021). Isolation and characterization of dominant lactic acid bacteria from raw goat milk: Assessment of probiotic potential and technological properties. Small Ruminant Research, 205, 106532. https://doi.org/https://doi.org/10.1016/j.smallrumres.2021.106532

Kinteki, G. A., Rizqiati, H., & Hintono, A. (2019). Pengaruh Lama Fermentasi Kefir Susu Kambing Terhadap Mutu Hedonik, Total Bakteri Asam Laktat (BAL), Total Khamir dan pH. Jurnal Teknologi Pangan, 3(1), 42–50. https://doi.org/https://doi.org/10.14710/jtp.2019.20685

Leinonen, I., Iannetta, P. P. M., Rees, R. M., Russell, W., Watson, C., & Barnes, A. P. (2019). Lysine Supply Is a Critical Factor in Achieving Sustainable Global Protein Economy . In Frontiers in Sustainable Food Systems (Vol. 3).

Li, X. Y., Cheng, M., Li, J., Zhao, X., Qin, Y. S., Chen, D., Wang, J. M., & Wang, C. F. (2020). Change in the structural and functional properties of goat milk protein due to pH and heat. Journal of Dairy Science, 103(2), 1337–1351. https://doi.org/https://doi.org/10.3168/jds.2019-16862

Matsui, Y., Takayanagi, S., Ohira, T., Watanabe, M., Murano, H., Furuhata, Y., & Miyakawa, S. (2019). Effect of a leucine-enriched essential amino acids mixture on muscle recovery. Journal of PHysical Therapy Science, 31(1), 95–101. https://doi.org/10.1589/jpts.31.95

Nurkhoeriyati, T., & Iswaldi, I. (2019). Peningkatan isoflavon dalam produk berbasis okara yang difermentasi oleh jamur. Jurnal Dunia Gizi, 2(1), 28–35. https://doi.org/10.33085/jdg.v2i1.4146

Pingitore, P., & Romeo, S. (2019). The role of PNPLA3 in health and disease. Biochimica et BiopHysica Acta (BBA) - Molecular and Cell Biology of Lipids, 1864(6), 900–906. https://doi.org/https://doi.org/10.1016/j.bbalip.2018.06.018

Priadi, G., Setiyoningrum, F., Afiati, F., Irzaldi, R., & Lisdiyanti, P. (2020). Studi in vitro bakteri asam laktat kandidat probiotik dari makanan fermentasi Indonesia. Jurnal Teknologi Dan Industri Pangan, 31(1), 21–28. https://doi.org/10.6066/jtip.2020.31.1.21

Puspita, L., & Komarudin, K. (2020). Peningkatan Ekonomi Masyarakat: Dampak Pemanfaatan Ampas Susu Kedelai Menjadi Nugget. Dinamisia : Jurnal Pengabdian Kepada Masyarakat, 5(1 SE-Articles). https://doi.org/10.31849/dinamisia.v5i1.4105

Rahman, M. M., Mat, K., Ishigaki, G., & Akashi, R. (2021). A review of okara (soybean curd residue) utilization as animal feed: Nutritive value and animal performance aspects. Animal Science Journal, 92(1), e13594. https://doi.org/https://doi.org/10.1111/asj.13594

Rahmaniar, R. P., Aini, D. W. N., Widyawati, R., & Mardjianto, A. (2021). Perbedaan kadar protein, kadar lemak dan nilai pH susu sapi pada daerah dataran tinggi dan dataran rendah di Kabupaten Jombang. Jurnal Sains Peternakan, 9(2), 100–103. https://doi.org/10.21067/jsp.v9i2.6271

Rochmah, S., Yani, A., & Aminarista. (2018). Kadar protein dan daya terima silky pudding yang disubstitusi susu kambing dan susu skim sebagai alternatif makanan tambahan balita stunting. Journal of Holistic and Health Sciences, 2(2), 72–77. https://doi.org/10.51873/jhhs.v2i2.29

Shariati, M. A. (2015). The Role of Research and Development in Agriculture and Its Dependent Concepts in Agriculture.

Susilawati, Zulferiyenni, Sari, P., & Sartika, D. (2017). Penyuluhan dan pelatihan pembuatan kefir susu kambing dalam rangka meningkatkan gizi masyarakat di Desa Sumber Rejo Kecamatan Jati Agung Kabupaten Lampung Selatan. Jurnal Sakai Sambayan, 1(3), 140–144.

Utami, R., Widowati, E., & Dewati, A. D. A. R. (2013). Kajian penggunaan tepung gembili (Dioscorea esculenta) dalam pembuatan minuman sinbiotik terhadap total bakteri probiotik, karakter mutu, dan karakter sensoris. Jurnal Teknosains Pangan, 2(3), 3–8.

Vong, W. C., & Liu, S. Q. (2016). Biovalorisation of okara (soybean residue) for food and nutrition. Trends in Food Science and Technology, 52, 139–147. https://doi.org/10.1016/j.tifs.2016.04.011

Yang, Q., Zhao, D., Zhang, C., Sreenivasulu, N., Sun, S. S.-M., & Liu, Q. (2022). Lysine biofortification of crops to promote sustained human health in the 21st century. Journal of Experimental Botany, 73(5), 1258–1267. https://doi.org/10.1093/jxb/erab482

Yoshida, B. Y., & Prudencio, S. H. (2020). PHysical, chemical, and technofunctional properties of okara modified by a carbohydrase mixture. LWT, 13(4), 110–141. https://doi.org/https://doi.org/10.1016/j.lwt.2020.110141

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