Efektivitas ekstrak daging buah pala (Myristica fragrans Houtt) terhadap histopatologi hati pada tikus hiperglikemia

Author(s): Amran Nur, Safitri Hasda Nur, Nur Asma S. Somadayo, Muhammad Fakhrur Rajih
DOI: 10.30867/gikes.v5i3B.1927

Abstract

Background: Hyperglycemia can occur due to insulin resistance, which affects the metabolism of lipids, carbohydrates, proteins, and fatty acids in the liver, which will ultimately cause cirrhosis, steatohepatitis, liver cancer, and other liver disorders.

Objectives: To determine the effect of administering nutmeg pulp extract (Myristica fragrans H.) on the histopathological picture of the liver of hyperglycemic mice induced by high carbohydrate feed.

Methods: The research was carried out using an experimental method using 25 white rats (Rattus norvegicus) of the Wistar strain, which were divided into five groups, K- (NaCMC 1%), K+ (Metformin), P1-P3 respectively Nutmeg extract 100mg/kgBW, 200mg/kgBB, 300mg/kgBB). The entire group was given high carbohydrate feed for 30 days, then extract and Metformin for seven days. On the 36th day, the liver was removed, and histopathology preparations were made. The main research outcome parameters include initial blood sugar levels before induction (T0), final day blood sugar levels (T30), blood sugar levels on days 1, 3, 5, and 7 after sample administration, liver weight, and liver severity score. The data obtained were analyzed using Oneway-Anova with a significance of 0.05, and LSD as a further test

Results: The K+, P1, and P3 groups showed a significant reduction in sugar compared to the control group (p<0.05), but all groups did not show an improvement in liver histopathology with damage scores in the mild to severe damage category.

Conclusion: Nutmeg pulp extract at doses of 100mg/kgBB, 200mg/kgBB, and 300mg/kgBB showed effectiveness in reducing blood sugar in hyperglycemic white mice but could not show optimal improvement in liver cell damage.

Keywords:

Diabetes mellitus, Histopatology, Liver, Myristica fragrans, Nutmeg Flesh.

Keywords

Diabetes mellitus, Histopatology, Liver, M.fragrans, Nutmeg Flesh.

Full Text:

PDF

References

Abdulkadir, W. S., Djuwarno, E. N., Ramadani Putri Papeo, D., & Marhaba, Z. (2023). Potensi ekstrak biji pala (myristica fragrans l) terhadap penyembuhan luka bakar pada mencit (mus musculus). Journal Syifa Sciences And Clinical Research, 5(1). Https://Doi.Org/10.37311/Jsscr.V5i1.18996

Dave, M., Gupta, A. K., Patel, P., & Heernath, H. (2019). Correlation between fasting blood sugar level, hba1c level and serum lipid levels in type 2 diabetes mellitus patients. International Journal Of Contemporary Medical Research [Ijcmr], 6(7). Https://Doi.Org/10.21276/Ijcmr.2019.6.7.13

Fatsecret. (2021, August 7). Nilai Gizi 200 Gram Nasi Putih. Fatsecret Platform Api.

Fitri, H., Maliza, R., Refiani, E., Laila, A., & Widyaningrum, M. (2021). Uji toksisitas ekstrak etanol kulit buah kopi arabika (coffea arabica l.) dan bawang dayak (eleutherine palmifolia l.) dengan metode brine shrimp lethality (bslt). Jurnal Galung Tropika, 10(2), 138–145. Https://Doi.Org/10.31850/Jgt.V10i2.751

Foretz, M., Guigas, B., & Viollet, B. (2023). Metformin: Update on mechanisms of action and repurposing potential. In Nature Reviews Endocrinology (Vol. 19, Issue 8, Pp. 460–476). Nature Research. Https://Doi.Org/10.1038/S41574-023-00833-4

Galicia-Garcia, U., Benito-Vicente, A., Jebari, S., Larrea-Sebal, A., Siddiqi, H., Uribe, K. B., Ostolaza, H., & Martín, C. (2020a). Pathophysiology Of Type 2 Diabetes Mellitus. In International Journal Of Molecular Sciences (Vol. 21, Issue 17, Pp. 1–34). Mdpi Ag. Https://Doi.Org/10.3390/Ijms21176275

Haryoto, H., & Devi, E. S. (2018). Efek pemberian ekstrak etanol daun dan batang ubi jalar ungu (ipomoea batatas l.) terhadap penurunan kadar glukosa darah pada tikus jantan galur wistar yang diinduksi aloksan. Talenta Conference Series: Tropical Medicine (Tm), 1(3), 139–143. Https://Doi.Org/10.32734/Tm.V1i3.279

International Diabetes Federation. (2021). Idf Diabetes Atlas 10th Edition (Vol. 10). Www.Diabetesatlas.Org

Jannah, D. R., & Budijastuti, W. (2022). Gambaran histopatologi toksisitas ginjal tikus jantan (rattus norvegicus) yang diberi sirup umbi yakon (Smallanthus Sonchifolius). Lenterabio : Berkala Ilmiah Biologi, 11(2), 238–246. Https://Doi.Org/10.26740/Lenterabio.V11n2.P238-246

Kementerian Kesehatan Republik Indonesia. (2017). Farmakope Herbal Indonesia Edisi Ii (11th Ed., Vol. 2).

Kuete, V. (2017). Myristica Fragrans: A Review. In Medicinal Spices And Vegetables From Africa: Therapeutic Potential Against Metabolic, Inflammatory, Infectious And Systemic Diseases (Pp. 497–512). Elsevier Inc. Https://Doi.Org/10.1016/B978-0-12-809286-6.00023-6

Kumar, A., Gangwar, R., Ahmad Zargar, A., Kumar, R., & Sharma, A. (2024). Prevalence Of Diabetes In India: A review of idf diabetes atlas 10th edition. Current Diabetes Reviews, 20(1). Https://Doi.Org/10.2174/1573399819666230413094200

Kumar Verma, N. (2021). Myristica Fragrans (Nutmeg): A Brief Review. Https://Doi.Org/10.36349/Easjpp.2021.V03i05.004

Lestari, Zulkarnain, & Sijid, St. A. (2021). Diabetes Melitus: Review etiologi, patofisiologi, gejala, penyebab, cara pemeriksaan, cara pengobatan dan cara pencegahan. Prosiding Biologi Achieving The Sustainable Development Goals With Biodiversity In Confronting Climate Change, 237–241. Http://Journal.Uin-Alauddin.Ac.Id/Index.Php/Psb

Madihah, M. (2017). Uji toksisitas akut ekstrak etanol kulit buah jengkol (archidendron pauciflorum) terhadap tikus wistar betina. In Prosiding Seminar Nasional Masyarakat Biodiversitas Indonesia. Masyarakat Biodiversitas Indonesia. Https://Doi.Org/10.13057/Psnmbi/M030107

Murtiningsih, M. K., Pandelaki, K., & Sedli, B. P. (2021). Gaya hidup sebagai faktor risiko diabetes melitus tipe 2. E-Clinic, 9(2), 328. Https://Doi.Org/10.35790/Ecl.V9i2.32852

Nur, A., Fiskia, E., & Rahman, I. (2022). Aktivitas antiinflamasi daging buah pala (myristica fragrans houtt.) pada tikus putih yang diinduksi karageenan. Jfionline | Print Issn 1412-1107 | E-Issn 2355-696x, 14(1), 10–16. Https://Doi.Org/10.35617/Jfionline.V14i1.87

Pasaribu, S. F., Chandra, P., Irwanto, R., Anggraini, C. D., & Herviana, H. (2023). Efek antidiabetes minuman gemar (germinated black rice) pada tikus model diabetes melitus. Journal Of Pharmaceutical And Sciences, 323–330. Https://Doi.Org/10.36490/Journal-Jps.Com.V6i5-Si.315

Rena, G., Hardie, D. G., & Pearson, E. R. (2017). The mechanisms of action of metformin. In Diabetologia (Vol. 60, Issue 9, Pp. 1577–1585). Springer Verlag. Https://Doi.Org/10.1007/S00125-017-4342-Z

Saputra, N. T., Suartha, I. N., & Dharmayudha, A. A. G. O. (2018). Agen diabetagonik streptozotocin untuk membuat tikus putih jantan diabetes mellitus. Buletin Veteriner Udayana, 116. Https://Doi.Org/10.24843/Bulvet.2018.V10.I02.P02

Seneme, E. F., Santos. Daiane C. D., Silva, E. M. R., Franco, Y. E. M., & Longato, G. B. (2021). Pharmacological and therapeutic potential of myristicin: A Literature Review. Molecules, 26(5914), 1–15. Https://Doi.Org/10.3390/Molecules

Tammuan, M., Pakaya, D., Salman, M., & Liwang, M. N. I. (2023). Efek metformin terhadap gambaran histopatologi otot rangka tikus model obesitas. Biomedika, 103–108. Https://Doi.Org/10.23917/Biomedika.V15i2.3303

DOI: http://dx.doi.org/10.30867/gikes.v5i3B.1927

Refbacks

  • There are currently no refbacks.