Efek sitotoksik ekstrak buah kapulaga jawa (Amomum compactum Soland. Ex. Maton)

Author(s): Nailul Muna, Noni Zakiah, Vonna Aulianshah, Munira Munira, Amelia Sari
DOI: 10.30867/gikes.v1i1.302

Abstract

Background: In developed countries cancer is the second leading cause of death after cardiovascular disease. Cancer prevention can be done with synthetic or traditional medicine. However, synthetic treatments often cause side effects and of course require high costs. Java Cardamom (Amomum compactum Soland. Ex Maton) is a plant that contains antioxidants. Antioxidants can inhibit oxidation reactions by binding to free radicals and molecules that are very reactive so that cell damage can be prevented. Cardamom contains flavonoids which can induce cell death programs as well as tannins as free radical scavengers.

Objectives: This study aims to determine the LC50 value of Java cardamom (Amomum compactum Soland. Ex Maton) on Artemia salina Leach.

Methods: This study was an experimental study using a completely randomized design (CRD) using 180 artemia larvae which were divided into 6 treatment groups. Each group included 10 artemia larvae with varying concentrations of 500 ppm, 250 ppm, 100 ppm, 50 ppm, 10 ppm and 0 ppm (not given extract). Three repetitions were performed for each concentration.

Results: The results showed from the observations obtained the LC50 value of Java cardamom extract is 26.60 ppm. So that the Java cardamom extract (Amomum compactum Soland. Ex Maton) has the potential as an anticancer (cytotoxic).

Conclusion: Java cardamom extract (Amomum compactum Soland. Ex Maton) is active and can be developed as an anticancer compound.

Keywords

Amomum compactum; BSLT; artemia salina leach

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References

American Cancer Society. (2019). Surveillance Research. In Cancer facts & Figures (p. 5).

B. N. Meyer., N. R. Ferrigni., J. E.Putnam., L. .B. Jacobsen., D. E.Nicols ., and J. L. M. (1982). Brine Shrimp : A Convenient General Bioassay for Active Plant Constituents. Journal of Mrdicinal Plant Research Planta Medika, 45(May 1982), 31–34. https://doi.org/10.1055/s-2007-971236

Das, A., Pal, K. K., & Nag, S. (2018). Anatomy, Micromorphology and Histochemical Localization of Different Phytochemicals of Two Medicinally Important Taxa of the Family Zingiberaceae. 4(191), 191–198. https://doi.org/10.26479/2018.0401.16

Gajardo, G. M., & Beardmore, J. A. (2012). The brine shrimp Artemia: adapted to critical life conditions. Frontiers in Physiology, 3, 185.

Geethaa, S. (2013). Interference from ordinarily used solvents in the outcomes of Artemia salina lethality test. 4(4), 179–182. https://doi.org/10.4103/2231-4040.121411

Gummadi, P. (2016). Role of Antioxidants on Cancer and Neurodegenerative Disorders. Journal of Medical & Health Science, 5(3), 1–6.

Hamidi, М. R., Jovanova, B., & Panovska, Т. K. (2014). Toxic оlogical evaluation of the plant products using Brine Shrimp ( Artemia salina L .) model. 60(1), 9–18.

Litaay, M et al. (2019). Toxicity of methanol fraction from marine tunicates Pyura sp and Polycarpa aurata to the brine shrimp Artemia salina Leach Toxicity of methanol fraction from marine tunicates Pyura sp and Polycarpa aurata to the brine shrimp Artemia salina. Earth and Enviromental Science 253 (2019)012014, 1–4. https://doi.org/10.1088/1755-1315/253/1/012014

Loprinzi, C., Bensinger, W., & Peterson, D. (2014). Understanding and managing chemotherapy side effects. 4–7.

Morshed, M. H., Das, P. K., Roy, A. K., & Ibrahim, M. (2018). Cytotoxicity of four active dyes against artemia salina leach. 09(2), 55–59.

Ogugu, S. E., Kehinde, A. J., James, B. I., & Paul, D. K. (2012). Assessment of cytotoxic effects of methanol extract of calliandra portoricensis using brine shrimp (artemia salina). Global Journal Of Bio-Science & Biotechnology, 1(2), 257–260.

World Health Organization. (2019). Indonesia Source GLOBOCAN 2018. International Agency for Research on Cancer, 256, 1–2.

DOI: http://dx.doi.org/10.30867/gikes.v1i1.302

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