Formulasi kombucha teh hijau tayu berbasis variasi jenis gula sebagai pangan fungsional: Analisis sensoris, kadar gula, dan aktivitas antioksidan
DOI: 10.30867/gikes.v7i1.3026Abstract
Background: Green tea and sugar are the main ingredients of kombucha. The antioxidant compounds in kombucha may play a role in the management of glucose metabolism. Using local Tayu green tea and a variety of sugars may potentially achieve the best kombucha characteristics for individuals with diabetes.
Objectives: This study aimed to identify the sensory characteristics, total sugar content, and antioxidant activity of tayu green tea kombucha fermented with different sugar types.
Methods: A Completely Randomized Design was used in this study. Three treatments based on the type of sugar in Tayu green tea kombucha were used: coconut sugar kombucha (GK), palm sugar kombucha (GA), and cane sugar kombucha (GT). Three kombucha samples were developed in the Pangkalpinang Laboratory and analyzed in the Bogor Laboratory from July to August 2025. The sensory characteristics were described based on product acceptability obtained from a hedonic test involving 30 panelists. The total sugar content was determined using the Luff Schoorl method, and the antioxidant activity was determined using the spectrophotometric method as IC50. Differences in acceptability data were analyzed using the Kruskal–Wallis test (p<0.05). Sugar content and antioxidant activity were analyzed univariately.
Results: Significant differences (p<0.05) in color and taste acceptability were observed, with the highest average observed for GK kombucha. The highest aroma and overall acceptability scores were observed for GT (4.3) and GK (4.6) samples. GK kombucha had the lowest IC50 value (6682.9mg/L), indicating the highest antioxidant activity because a lower concentration was required to neutralize 50% of free radicals. GA kombucha had the lowest sugar content at 12.1%, and GK had a slightly higher sugar content at 13.3%.
Conclusion: GTK with coconut sugar is the best formula for DM, with the highest acceptability value, moderate sugar content, and highest antioxidant activity.
Keywords
Full Text:
PDFReferences
Al-Khalili, M., Pathare, P. B., Rahman, S., & Al-Habsi, N. (2025). Aroma compounds in food: Analysis, characterization and flavor perception. Measurement: Food, 18(1), 1–4. https://doi.org/10.1016/j.meafoo.2025.100220
Alkhatib, A., Tsang, C., Tiss, A., Bahorun, T., Arefanian, H., Barake, R., Khadir, A., & Tuomilehto, J. (2017). Functional foods and lifestyle approaches for diabetes prevention and management. Nutrients, 9(12), 1–18. https://doi.org/10.3390/nu9121310
Bishop, P., Pitts, E. R., Budner, D., & Thompson-Witrick, K. A. (2022). Kombucha: Biochemical and microbiological impacts on the chemical and flavor profile. Food Chemistry Advances, 1(1), 1–9. https://doi.org/10.1016/j.focha.2022.100025
Cavicchia, L. O. A., & de Almeida, M. E. F. (2022). Health benefits of Kombucha: drink and its biocellulose production. Braz. J. Pharm, 58(1), 1–3. https://doi.org/10.1590/s2175-97902022e20766
Cohen, G., Sela, D. A., & Nolden, A. A. (2023). Sucrose Concentration and Fermentation Temperature Impact the Sensory Characteristics and Liking of Kombucha. Foods, 12, 1–13. https://doi.org/10.3390/foods12163116
Djeya Joelle, O., Konan Jean Louis, K., DMurielJ, O., JeanLouis, K. K., & RebeccaR, A. (2020). Sensory Properties of Table Sugars Derived from the Inflorescences Sap of Three Coconut (Cocos Nucifera.L) Cultivars in Côte d’Ivoire "Sensory Properties of. American Journal of Food and Nutrition, 8(3), 90–100. https://doi.org/10.12691/ajfn-8-3-6
Dwiloka, B., Rizqiati, H., Adiwiratna, P. L., Hapsari, A. E. K., & Dewi, N. S. (2024). Physicochemical Characteristics of Bitter Leaf (Vernonia amygdalina Del.) Kombucha with Palm Sugar Addition. Journal of Applied Food Technology, 11(1), 20–26. https://doi.org/10.17728/jaft.22187
El Hosry, L., Elias, V., Chamoun, V., Halawi, M., Cayot, P., Nehme, A., & Bou-Maroun, E. (2025). Maillard Reaction: Mechanism, Influencing Parameters, Advantages, Disadvantages, and Food Industrial Applications: A Review. Foods, 14(11), 1–43. https://doi.org/10.3390/foods14111881
Filbert, J Koleangan, H. S., J Runtuwene, M. R., & Kamu, V. S. (2014). Penentuan Aktivitas Antioksidan Berdasarkan Nilai IC 50 Ekstrak Metanol dan Fraksi Hasil Partisinya pada Kulit Biji Pinang Yaki (Areca vestiaria Giseke). Jurnal MIPA Unsrat, 3(2), 149–154.
International Diabetes Federation. (2025). 11th edition Diabetes Atlas.
Jakubczyk, K., Kałduńska, J., Kochman, J., & Janda, K. (2020). Chemical profile and antioxidant activity of the kombucha beverage derived from white, green, black and red tea. Antioxidants, 9(5), 1–15. https://doi.org/10.3390/antiox9050447
Jayanudin, J., Kurniawan, T., & Kustiningsih, I. (2019). Phenolic Analysis and Characterization of Palm Sugar (Arenga pinnata) Produced by The Spray dryer. Oriental Journal of Chemistry, 35(1), 150–156. https://doi.org/10.13005/ojc/350116
Jiang, Q., Wang, S., Yang, Y., Luo, J., Yang, R., & Li, W. (2023). Profiles of Free and Bound Phenolics and Their Antioxidant Capacity in Rice Bean (Vigna umbellata). Foods, 12(14), 1–15. https://doi.org/10.3390/foods12142718
Kemenkes RI, B. K. P. K. (2024). Survey Kesehatan Indonesia Dalam Angka (Vol. 1).
Kiczorowski, P., Kiczorowska, B., Samolińska, W., Szmigielski, M., & Winiarska-Mieczan, A. (2022). Effect of fermentation of chosen vegetables on the nutrient, mineral, and biocomponent profile in human and animal nutrition. Scientific Reports, 12(1), 1–13. https://doi.org/10.1038/s41598-022-17782-z
Kurniawan, T., Jayanudin, J., Kustiningsih, I., & Adha Firdaus, M. (2018). Palm Sap Sources, Characteristics, and Utilization in Indonesia. Journal of Food and Nutrition Research, 6(9), 590–596. https://doi.org/10.12691/jfnr-6-9-8
M. Shende, S. (2024). “Bioactive Compounds in Diabetes Management: Antidiabetic Properties and Therapeutic Potential.” African Journal of Biomedical Research, 27(3s), 2303–2310. https://doi.org/10.53555/AJBR.v27i3S.2615
Maghfirah, D., Fithri Pulungan, A., Ridwanto, & Yuniarti, R. (2025). Comparison of Phenolic and Antioxidant Contents In Tea Breawing and Kombucha Tea Variants by Visible Spectrophotometry. Journal of Pharmaceutical and Sciences, 8(3), 1384–1397.
Mariana. (2017). Kadar Polifenol Daun Teh yang Ditanam di Dusun Tayu Desa Ketap Kecamatan Jebus Kabupaten Bangka Barat [Universitas Bangka Belitung]. http://repository.ubb.ac.id/1521/1/MARIANAc %282031211013%29_cover_1.pdf
Maryani, Y., Rochmat, A., Khastini, R. O., Kurniawan, T., & Saraswati, I. (2021). Identification of Macro Elements (Sucrose, Glucose and Fructose) and Micro Elements (Metal Minerals) in the Products of Palm Sugar, Coconut Sugar and Sugar Cane.
Mihafu, F. D., Issa, J. Y., & Kamiyango, M. W. (2020). Implication of sensory evaluation and quality assessment in food product development: A review. Current Research in Nutrition and Food Science, 8(3), 690–702. https://doi.org/10.12944/CRNFSJ.8.3.03
Muhialdin, B. J., Muhamad, R., Anzian, A., & Voon, W. (2019). Effects of sugar sources and fermentation time on the properties of tea fungus (kombucha) beverage. International Food Research Journal, 26(2), 481–487.
Nascimento, V. M., Antoniolli, G. T. U., Leite, R. S. R., & Fonseca, G. G. (2020). Effects of the carbon source on the physiology and invertase activity of the yeast Saccharomyces cerevisiae FT858. 3 Biotech, 10(8), 1–9. https://doi.org/10.1007/s13205-020-02335-w
Novidiyanto, & Sutyawan. (2022). Chemical Characteristic of Green Tea “Tayu” from Bangka Belitung Province and Green Tea of Commersial. Jurnal Gizi Dan Kesehatan (JGK), 2(1), 74–81. https://doi.org/10.36086/jgk.v2i1
Perli, T., Wronska, A. K., Ortiz-Merino, R. A., Pronk, J. T., & Daran, J. M. (2020). Vitamin requirements and biosynthesis in Saccharomyces cerevisiae. Yeast, 37(4), 283–304. https://doi.org/10.1002/yea.3461
Santos, S. C., Fortes, G. A. C., Camargo, L. T. F. M., Camargo, A. J., & Ferri, P. H. (2021). Antioxidant effects of polyphenolic compounds and structure-activity relationship predicted by multivariate regression tree. LWT - Food Science and Technology, 137, 1–4. https://doi.org/10.1016/j.lwt.2020.110366
Saraiva, A., Carrascosa, C., Ramos, F., Raheem, D., Lopes, M., & Raposo, A. (2023). Coconut Sugar: Chemical Analysis and Nutritional Profile; Health Impacts; Safety and Quality Control; Food Industry Applications. Int. J. Environ. Res. Public Health, 20(4), 1. https://doi.org/10.3390/ijerph20043671
Sarkar, T., Mukherjee, M., Roy, S., & Chakraborty, R. (2023). Palm sap sugar an unconventional source of sugar exploration for bioactive compounds and its role on functional food development. Heliyon 9, 9(4), 1–18. https://doi.org/10.1016/j.heliyon.2023.e14788
Sarpong, F., Anning, D., & Oduro-Yeboah, C. (2024). Trends in Coconut Brown Sugar Production – A Review of Health and Future Prospect in the Industry. Turkish Journal of Agriculture - Food Science and Technology, 12(s2), 2407–2414. https://doi.org/10.24925/turjaf.v12is2.2407-2414.6907
Seguí, L., Calabuig-Jiménez, L., Betoret, N., & Fito, P. (2015). Physicochemical and antioxidant properties of non-refined sugarcane alternatives to white sugar. International Journal of Food Science and Technology, 50(12), 2579–2588. https://doi.org/10.1111/ijfs.12926
Sipahutar, A. S., Elwina, E., & Zulkifli, Z. (2024). Pengaruh Jenis Gula Dan Waktu fermentasi Terhadap Kualitas Minuman Fermentasi Kombucha Air Kelapa. Jurnal Ristera, (2), 53–57.
Soltan, M., Elsamadony, M., Mostafa, A., Awad, H., & Tawfik, A. (2019). Nutrients balance for hydrogen potential upgrading from fruit and vegetable peels via fermentation process. Journal of Environmental Management, 242, 384–393. https://doi.org/10.1016/j.jenvman.2019.04.066
Sutyawan, & Novidiyanto. (2022). Perbandingan Sifat Sensoris dan Kadar Total Fenol pada Teh Hitam Kombucha dari Daun Teh Tayu dan Teh Hitam Komersil. Jurnal Kesehatan Poltekkes Kemenkes RI Pangkalpinang, 10(1), 49–56. https://doi.org/10.32922/jkp.v10i1.439
Sutyawan, S., & Novidiyanto, N. (2021). Analisis Perbedaan Karakteristik Kimia Dan Aktivitas Antioksidan Pada Teh Kombucha Hitam Dari Daun Tayu Dan Teh Komersil. Pontianak Nutrition Journal (PNJ), 4(2), 112–118. https://doi.org/10.30602/pnj.v4i2.905
Swasti, Y. R., Purwijantiningsih, E., & Pranata, F. S. (2024). Antioxidant activities and mutagenic compounds in coconut and palm sugar from special region of Yogyakarta Indonesia. Food Research, 8(4), 336–342. https://doi.org/10.26656/fr.2017.8(4).251
Velasco, C., Barbosa Escobar, F., Spence, C., & Olier, J. S. (2023). The taste of colours. Food Quality and Preference, 112(1), 12. https://doi.org/10.1016/j.foodqual.2023.105009
Wahyuni, T., Rusmawan, D., & Muzammil. (2021). Eksplorasi Dan Karakterisasi Teh Tayu (Camellia sinensis L.) di Kabupaten Bangka Barat. In N. Hidayatun & L. Herlina (Eds.), Prosiding Seminar Nasional Komisi Nasional Sumber Daya Genetik (pp. 586–595). Komisi Nasional Sumber Daya Genetik.
Wang, B., Rutherfurd-Markwick, K., Zhang, X. X., & Mutukumira, A. N. (2022). Kombucha: Production and Microbiological Research †. Foods, 11(21), 1–18. https://doi.org/10.3390/foods11213456
Wrage, J., Burmester, S., Kuballa, J., & Rohn, S. (2019). Coconut sugar (Cocos nucifera L.): Production process, chemical characterization, and sensory properties. LWT-Food Science And Technology, 112, 1–6. https://doi.org/10.1016/j.lwt.2019.05.125
Yameny, A. A. (2024). Diabetes Mellitus Overview 2024. Journal of Bioscience and Applied Research, 10(3), 641–645. https://doi.org/10.21608/jbaar.2024.382794
Refbacks
- There are currently no refbacks.











