Efek diet tinggi lemak terhadap berat testis dan kadar glukosa darah puasa tikus Wistar jantan

Author(s): Rista Dwi Hermilasari, Septy Handayani, Ellanda Rima Ervyanna
DOI: 10.30867/gikes.v7i2.3138

Abstract

Background: Globally, modern lifestyle trends are characterized by increased consumption of high-fat food, which contribute to metabolic disorders. High-fat diets can also cause disorders in the male reproductive system. However, evidence regarding the association between changes in testicular weight and glycemic dysregulation as indicators of metabolic-reproductive disorders in animal studies remains limited.

Objectives: This study aimed to evaluate the effect of high-fat diet on testicular weight and fasting blood glucose levels inmale Wistar rats.

Methods: This study was conducted in June toJuly 2024 at the Basic Dentistry Laboratory, University of Jember using a randomized posttest-only control group design. A total of 14 male Wistar rats were divided into two groups: P1 (normal diet) and group P2 consisting of 2 ml of egg yolk given for 28 days. The rats were provided with drinking water ad libitum. Fasting blood glucose levels were measured using an Easy Touch GCU glucometer. After the rats were euthanized and dissected, testicular weight was measured using a digital scale with an accuracy of 0,1 g. Data were analyzed using an independent t-test with a significance value of p < 0.05.

Results: Administration of a high-fat diet to male Wistar rats showed lower testicular weight compared with the normal diet group, but the difference was not statistically significant (p = 0.315).In contrast , administration of high-fat diet to Wistar rats showed a significant difference in fasting blood glucose levels compared the normal diet group (p < 0.001).

Conclusion: A high-fat diet significantly increases fasting blood glucose levels, indicating impaired glucose homeostasis and potentially contributing to changes in testicular weight, which may disrupt reproductive function.

Keywords

High-fat diet, fasting blood glucose, reproduction, Wistar rats, testis

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References

Abbasi, E., & Khodadadi, I. (2025). High-fat diet may increase the risk of insulin resistance by inducing dysbiosis. Metabol Open, 27, 100381. https://doi.org/10.1016/j.metop.2025.100381

Adthapanyawanich, K., Aitsarangkun, K., Ayutthaya, N., Kreungnium, S., Mark, P. J., Nakata, H., Chen, W., Chinda, K., Amatyakul, P., & Tongpob, Y. (2025). Molecular Mechanisms and Therapeutic Potential of Mulberry Fruit Extract in High-Fat Diet-Induced Male Reproductive Dysfunction: A Comprehensive Review. Nutrients, 17(2), 273.

Ahmed, S. K., & Mohammed, R. A. (2025). Obesity: Prevalence, causes, consequences, management, preventive strategies and future research directions. Metabolism Open, 27, 100375. https://doi.org/10.1016/j.metop.2025.100375

Barber, T. M., Kabisch, S., & Pfeiffer, A. F. H. (2024). Dietary and Lifestyle Strategies for Obesity. Nutrients, 16(16), 2714.

Bhagtani, D., Adams, J., Imamura, F., Lahiri, A., Pradeepa, R., Mahmood, S., Shamim, A. A., Akter, F., Loomba, M., Athauda, L., De Silva, L., Khawaja, K. I., Ahmad, S., Jha, V., Kasturiratne, A., Katulanda, P., Mridha, M. K., Anjana, R. M., Chambers, J. C., & Forouhi, N. G. (2025). Quantification of regional variation in ultra-processed food consumption and its sociodemographic correlates across Bangladesh, India, Pakistan, and Sri Lanka: insights from the South Asia Biobank. Lancet Reg Health Southeast Asia, 39, 100633. https://doi.org/10.1016/j.lansea.2025.100633

Billah, M. M., Khatiwada, S., Lecomte, V., Morris, M. J., & Maloney, C. A. (2022). Ameliorating high-fat diet-induced sperm and testicular oxidative damage by micronutrient-based antioxidant intervention in rats. Eur J Nutr, 61(7), 3741–3753. https://doi.org/10.1007/s00394-022-02917-9

Budi, Y. P., Li, Y.-H., Huang, C., Wang, M.-E., Lin, Y.-C., Jong, D.-S., Chiu, C.-H., & Jiang, Y.-F. (2022). The role of autophagy in high-fat diet-induced insulin resistance of adipose tissues in mice. PeerJ, 10, e13867. https://doi.org/10.7717/peerj.13867

Campos, I. X., Duarte, M., Mishima, V., Milagro, F. I., & Gouveia, C. (2025). A Low-Cost, High-Fat Diet Effectively Induces Obesity and Metabolic Alterations and Diet Normalization Modulates Microbiota in C57BL/ 6 Mice. Nutrients, 17(23), 3806.

Crean, A. J., & Senior, A. M. (2019). High‐fat diets reduce male reproductive success in animal models: A systematic review and meta‐analysis. Obes Rev, 20(6), 921–933. https://doi.org/10.1111/obr.12827

Dewistamara, M. G., Qomariyah, N., & Khaleyla, F. (2025). Effect of Different Types of High-Fat Diet (HFD) for Induction of Type 2 Diabetes Mellitus in Animal Model. LenteraBio, 14(3), 331–339.

Falvo, S., Minucci, S., Santillo, A., Senese, R., & Chief, G. (2023). A short-term high-fat diet alters rat testicular activity and blood-testis barrier integrity through the SIRT1/ NRF2/ MAPKs signaling pathways. Front Endocrinol, 14, 1274035. https://doi.org/10.3389/fendo.2023.1274035

Hafez, M. H., Gad, S. B., & El-sayed, Y. S. (2025). Quercetin-mediated restoration of high-fat diet-induced male reproductive dysfunction through modifying spermatogenesis and unraveling 3β-HSD, 17β-HSD, and StAR pathways. BMC Pharmacol Toxicol, 26(1), 90.

Hu, S., Wang, L., Wu, Y., Yang, D., Xu, Y., Li, L., Li, B., Li, M., Li, J., Wang, G., Zhang, X., Niu, C., Mazidi, M., & Douglas, A. (2021). Effects of dietary macronutrients and body composition on glucose homeostasis in mice. Natl Sci Rev, 8(1), 177. https://doi.org/10.1093/nsr/nwaa177

Kang, Y., Li, P., Yuan, S., Fu, S., Zhang, X., Zhang, J., Dong, C., & Xiong, R. (2025). Progress in Investigating the Impact of Obesity on Male Reproductive Function. Biomedicines, 13(9), 2054.

Lei, C., Wang, J., Li, X., Mao, Y., & Yan, J. (2023). Changes of insulin receptors in high fat and high glucose diet mice with insulin resistance. Adipocyte, 12(1), 1–10. https://doi.org/10.1080/21623945.2023.2264444

Leisegang, K., Sengupta, P., Agarwal, A., & Henkel, R. (2021). Obesity and male infertility: Mechanisms and management. Andrologia, 53(1), e13617. https://doi.org/10.1111/and.13617

Maleki, A. H., Azar, J. T., Razi, M., & Tofighi, A. (2024). The Effect of Different Exercise Modalities on Sertoli-germ Cells Metabolic Interactions in High-fat Diet-induced Obesity Rat Models: Implication on Glucose and Lactate Transport, Igf1, and Igf1R-dependent Pathways. Reprod Sci, 31(8), 2246–2260. https://doi.org/10.1007/s43032-024-01533-8

Marino, M., Puppo, F., Del Bo’, C., Vinelli, V., Riso, P., Porrini, M., & Martini, D. (2021). A Systematic Review of Worldwide Consumption of Ultra-Processed Foods: Findings and Criticisms. Nutrients, 13(8), 2778. https://doi.org/10.3390/nu13082778

Mengr, A., Strnadová, V., Strnad, S., Vrkoslav, V., Pelantová, H., Kuzma, M., Comptdaer, T., Železná, B., Kuneš, J., Galas, M., Pacesová, A., & Maletínská, L. (2023). Feeding High-Fat Diet Accelerates Development of Peripheral and Central Insulin Resistance and Inflammation and Worsens AD-like Pathology in APP/PS1 Mice. Nutrients, 15(17), 3690.

Mo, Q., Deng, X., Zhou, Z., & Yin, L. (2025). High-Fat Diet and Metabolic Diseases: A Comparative Analysis of Sex-Dependent Responses and Mechanisms. Int J Mol Sci, 26(10), 4777.

Park, J., Yoo, J., & Han, J. (2024). HM-Chromanone Alleviates Hyperglycemia by Activating AMPK and PI3K / AKT Pathways in Mice Fed a High-Fat Diet. Nutrients, 16(22), 3972.

Retiaty, F., Andarwulan, N., Palupi, N. S., Ernawati, F., & Kazimierczak, R. (2025). Contribution of Food , Energy, Macronutrients and Fiber Consumption Patterns to Obesity and Other Non-Communicable Disease Risks in the Indonesian Population. Nutrients, 17(9), 1459.

Service, C. A., Puri, D., Azzawi, S. Al, Hsieh, T., & Patel, D. P. (2023). The impact of obesity and metabolic health on male fertility: a systematic review. Fertil Steril, 120(6), 1098–1111. https://doi.org/10.1016/j.fertnstert.2023.10.017

Uddandrao, V. V. S., Eraniappan, S., Balakrishnan, A., Singaravel, S., Roy, A., Parim, B. N., Ponnusamy, C., Ganapathy, S., & Sasikumar, V. (2024). Systems Biology in Reproductive Medicine Hydroxycitric acid and capsaicin combination alleviates obesity-induced testicular apoptosis, oxidative stress and inflammation. Syst Biol Reprod Med, 70(1), 20–37. https://doi.org/10.1080/19396368.2024.2306403

Vásquez, B., Leviman, B., & Garcia, L. (2025). Testicular Vulnerability to Early-life Stress and High-fat Diet. Int J Morphol, 43(5), 1796–1800.

Wali, J. A., Ni, D., Facey, H. J. W., Dodgson, T., Pulpitel, T. J., Senior, A. M., Raubenheimer, D., Macia, L., & Simpson, S. J. (2023). Determining the metabolic effects of dietary fat, sugars and fat-sugar interaction using nutritional geometry in a dietary challenge study with male mice. Nature Communications, 14, 4409. https://doi.org/10.1038/s41467-023-40039-w

Wang, J., Dai, L., Yu, T., & Id, J. X. (2025). Insights into the progressive impact of high- fat-diet induced insulin resistance on skeletal muscle and myocardium: A comprehensive study on C57BL6 mice. PLoS One, 20(1), e0310458. https://doi.org/10.1371/journal.pone.0310458

Wang, L., Wang, H., Zhang, B., Popkin, B. M., & Du, S. (2020). Elevated Fat Intake Increases Body Weight and the Risk of Overweight and Obesity among Chinese Adults: 1991-2015 Trends. Nutrients, 12(11), 3272. https://doi.org/10.3390/nu12113272

Xie, D., Zhang, Y., Guo, Y., Xue, X., Zhao, S., Geng, C., Li, Y., Yang, R., Gan, Y., Li, H., Ren, Z., & Jiang, P. (2023). The impact of high-glucose or high-fat diets on the metabolomic profiling of mice. Front Nutr, 10, 1171806. https://doi.org/10.3389/fnut.2023.1171806

Yi, X., Tang, D., Cao, S., Li, T., Gao, H., Ma, T., Yao, T., Li, J., & Chang, B. (2020). Effect of Different Exercise Loads on Testicular Oxidative Stress and Reproductive Function in Obese Male Mice. Oxid Med Cell Longev, 2020, 3071658.

Zhang, W., Tian, Z., Qi, X., & Chen, P. (2023). Switching from high ‑ fat diet to normal diet ameliorate BTB integrity and improve fertility potential in obese male mice. Sci Rep, 13(1), 14152. https://doi.org/10.1038/s41598-023-41291-2

Zhang, Y., Wang, R., & Liu, T. (2024). Exercise as a Therapeutic Strategy for Obesity: Central and Peripheral Mechanisms. Metabolites, 14(11), 589.

DOI: http://dx.doi.org/10.30867/gikes.v7i2.3138

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